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RNA extraction using trizol/tri: Difference between revisions
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→cell lysis: icon
(→RNA wash: icon) |
(→cell lysis: icon) |
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* (5min at RT for complete dissociation of nucleoprotein complexes) | * (5min at RT for complete dissociation of nucleoprotein complexes) | ||
[[Image:Pause point.png]] RNA is stable in trizol which deactivates RNases. You can take a break at this point keeping the sample in trizol for a short time or freezing it for a longer one. | |||
=== phase separation === | === phase separation === | ||
