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TOP10 chemically competent cells: Difference between revisions
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TOP10 chemically competent cells (view source)
Revision as of 09:49, 11 February 2008
, 11 February 2008→5x Ligation Adjustment Buffer
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==5x Ligation Adjustment Buffer== | ==5x Ligation Adjustment Buffer== | ||
* Intended to be mixed with ligation reactions to adjust buffer composition to be near the CCMB80 buffer | * Intended to be mixed with ligation reactions to adjust buffer composition to be near the CCMB80 buffer | ||
* KOAc 40 mM (40 ml/liter of 1 M KOAc solution, pH 7.0) | * KOAc 40 mM (40 ml/liter of 1 M KOAc solution, pH 7.0) | ||
| Line 90: | Line 89: | ||
* autoclave or sterile filter | * autoclave or sterile filter | ||
* Test pH adjustment by mixing 4 parts ligation buffer + 1 part 5x ligation adjustment buffer and checking pH to be 6.3 - 6.5 | * Test pH adjustment by mixing 4 parts ligation buffer + 1 part 5x ligation adjustment buffer and checking pH to be 6.3 - 6.5 | ||
*'''[[User:Reshma P. Shetty|Reshma]] 10:49, 11 February 2008 (CST)''': Use of the ligation adjustment buffer is optional. | |||
==References== | ==References== | ||
