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Choosing reference genes for qPCR normalisation: Difference between revisions
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Choosing reference genes for qPCR normalisation (view source)
Revision as of 11:31, 7 February 2008
, 7 February 2008→Reference genes across tissues
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== Reference genes across tissues == | == Reference genes across tissues == | ||
If you are comparing mRNA/cDNA levels from different tissue it is especially important that reference gene levels are close to constant across different tissues. Radonić et al compared 13 putative reference gene levels in 13 different human tissues [PMID 14706621]. The results are summarised below | If you are comparing mRNA/cDNA levels from different tissue it is especially important that reference gene levels are close to constant across different tissues. Radonić et al compared 13 putative reference gene levels in 13 different human tissues [PMID 14706621]. The results are summarised below: | ||
* genes with the smallest | * genes with the smallest range (most constant levels): '''TBP''', '''RP2''', Act, Tub, PLA | ||
* genes | * genes with the largest range (unsuitable for cross-tissue comparison): HPRT, Alb, PBGD, GAPDH, β2M | ||
* genes undetectable in tissue: Alb - colon; PPIA - ovaries; HPRT - prostate, testis, ovary, small intestine, colon; PBL, skeletal muscle; Tub - ovaries, PBGD - skeletal muscle; TBP - lung, prostate | |||
* genes detected in all tissues: GAPDH, Act, β2M, L13, PLA, G6PDH, RP2 | |||
(note the source Fig 2 is sometimes impossible to read and the describing text is incomplete; that might have lead to some errors above) | |||
== Primer collections == | == Primer collections == | ||
