2,879
edits
Choosing reference genes for qPCR normalisation: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Choosing reference genes for qPCR normalisation (view source)
Revision as of 10:20, 6 February 2008
, 6 February 2008→The ideal reference gene
(normalisation) |
|||
| Line 14: | Line 14: | ||
Common reference mRNAs linked to known mouse primer pairs: | Common reference mRNAs linked to known mouse primer pairs: | ||
* β-actin (common cytoskeletal enzyme) [http://medgen.ugent.be/rtprimerdb/search_results.php?id=&application=-1&organism=2&gene=actin&string_type=substring&detection=-1&primer=&locuslink=&snp=&last_name=&pubmed=&order=0&search=Search+Primers&first_result=0], [http://www.realtimeprimers.org/SYBR%20Green/Mouse%20SYBR%20Green.html] | * β-actin (common cytoskeletal enzyme) [http://medgen.ugent.be/rtprimerdb/search_results.php?id=&application=-1&organism=2&gene=actin&string_type=substring&detection=-1&primer=&locuslink=&snp=&last_name=&pubmed=&order=0&search=Search+Primers&first_result=0], [http://www.realtimeprimers.org/SYBR%20Green/Mouse%20SYBR%20Green.html] | ||
* ribosomal proteins (e.g. RPLP0) | |||
* ribosomal proteins (e.g. RPLP0 | |||
* cyclophilin mRNA | * cyclophilin mRNA | ||
* MHC I (major histocompatibility complex I) | * MHC I (major histocompatibility complex I) | ||
Common but not recommended references | |||
* glyceraldehyde-3-phosphate dehydrogenase GAPDH (common metabolic enzyme) [http://medgen.ugent.be/rtprimerdb/search_results.php?primer=&application=-1&detection=-1&locuslink=&snp=&last_name=&order=0&search=Search+Primers&first_result=0&pubmed=&string_type=substring&gene=gapdh&organism=2&Search=Search&id=], [http://www.realtimeprimers.org/SYBR%20Green/Mouse%20SYBR%20Green.html] - see [[QRT-PCR#Reference_mRNAs]] | |||
* ribosomal RNAs (28S or 18S) - see below | |||
Search primer repositories like [http://medgen.ugent.be/rtprimerdb/ RTPrimerDB] (see also below) and check the literature before doing it from scratch. <br> | Search primer repositories like [http://medgen.ugent.be/rtprimerdb/ RTPrimerDB] (see also below) and check the literature before doing it from scratch. <br> | ||
