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| ==''Escherichia coli''== | | ==''Escherichia coli''== |
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| ===Information===
| | See [[Escherichia coli/Vectors]]. |
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| *[[Bacterial artificial chromosomes]]
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| *[[F plasmids]]
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| *[[Synthetic Biology:Vectors | Synthetic Biology vectors]]
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| ====Common vectors====
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| {|border="1"
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| |-
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| |Plasmid
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| |Replicon
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| |Copy number
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| |-
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| |pBR322<cite>Bolivar-Gene-1977</cite> and its derivatives
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| |pMB1
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| |15-20
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| |-
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| |pUC vectors
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| |pMB1
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| |500-700
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| |-
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| |pACYC and its derivatives
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| |p15A<cite>Chang-JBacteriol-1978</cite>
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| |10-12
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| |-
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| |pSC101 and its derivatives
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| |pSC101<cite>Cohen-JBacteriol-1977</cite>
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| |5
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| |-
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| |ColE1
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| |ColE1<cite>Bazaral-J-Mol-Biol-1968,Hershfield-Proc-Natl-Acad-Sci-USA-1974</cite>
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| |15-20
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| |}
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| ===Replicon Compatibility===
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| {| border="1" cellpadding="4" cellspacing="0" style="border:#c9c9c9 1px solid; margin: 1em 1em 1em 0; border-collapse: collapse; width:710px" <!-- This line here formats your table for you. Change the code to change the formatting of your table.-->
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| | align="center" style="background:#f0f0f0;"|'''Incompatibility group'''
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| | align="center" style="background:#f0f0f0;"|'''Negative control element'''
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| | align="center" style="background:#f0f0f0;"|'''Comment'''
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| |-
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| | colE1<cite>Bazaral-J-Mol-Biol-1968,Hershfield-Proc-Natl-Acad-Sci-USA-1974</cite>, pMB1
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| | RNAI
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| | controls processing of pre-RNAII into primer. pUC is derived from pBR322 (a single mutation in the pBR322 Primer RNA and deletion of the rop gene) which is derived from a pMB1 replicon, and cannot co-reside with the colE1 incompatibility group.
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| |-
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| | IncFII, pT181
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| | RNA
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| | controls synthesis of RepA protein
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| |-
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| | P1, F, R6K, pSC101<cite>Cohen-JBacteriol-1977</cite>, p15A<cite>Chang-JBacteriol-1978</cite>
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| | iterons
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| | sequesters RepA protein
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| |}
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| Replication origins in different incompatibility groups are compatible. Replication origins in the same incompatibility group are not.
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| Some sets of vectors with compatible origins are available as a part of the [http://www.emdbiosciences.com/html/NVG/DuetTable.html Novagen Duet system]. (from [[User:Tk | TK]])
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| ====Reference====
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| <biblio>
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| #MolecularCloning isbn=0-87969-577-3
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| #Anderson-Annu-Rev-Microbiol-1968 pmid=4879515
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| // Discusses transferable drug resistance in ''Escherichia coli'' (i.e. plasmids with resistance markers)
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| #Cohen-Proc-Natl-Acad-Sci-USA-1973a pmid=4576014
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| // Discusses generation of plasmids by sharing DNA, their transformation and replication.
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| #Cohen-Proc-Natl-Acad-Sci-USA-1973b pmid=4594039
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| // Discusses use of vector to propagate other DNA fragments
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| #Chang-JBacteriol-1978 pmid=149110
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| // p15A origin
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| #Cohen-JBacteriol-1977 pmid=334752
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| // pSC101 origin
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| #Bolivar-Gene-1977 pmid=344137
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| // vector pBR322
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| #Bazaral-J-Mol-Biol-1968 pmid=4939624
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| //original discovery of ColE1 plasmid
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| #Hershfield-Proc-Natl-Acad-Sci-USA-1974 pmid=4610576
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| // use of ColEI as a vector
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| </biblio>
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| ===Individual vector links===
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| ''Note: searching for cloning vector <insert vector name> when looking for vector sequences in NCBI Entrez Nucleotide search. It helps to cut down on the number of hits.''
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| *[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=6691170 pUC19]
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| *[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=1817728 pBeloBAC11]
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| *[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=4878025 pBACe3.6]
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| *[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=9507713 F plasmid]
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| *pSCANS [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=Nucleotide&cmd=Retrieve&list_uids=6689911&dopt=GenBank genbank] [http://genome.bnl.gov/Vectors/pscans.php vector info] [http://www.genome.bnl.gov/Protocols/pscans.shtml cookbook]
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| *[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=42704 pSC101 replication origin]
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| ===Other references===
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| *[http://www.emdbiosciences.com/g.asp?f=NVG/pETtable.html Novagen pET vector table]
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| **table with links to properties/sequences of pET vectors
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| **note: slow to load
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| ==Yeast== | | ==Yeast== |
