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Annealing complementary primers (view source)
Revision as of 15:02, 10 January 2006
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==Introduction== | ==Introduction== | ||
A simple and cheap way to make a short (< 100 bp) piece of DNA is to order two complementary primers from a company such as [http://www.invitrogen.com Invitrogen]. Other options are described under [[Synthetic Biology:BioBricks/Part fabrication | part fabrication]] and [[Annealing and primer extension|annealing and primer extension]]. Having annealed the primers, you most likely want to use them as an insert into a [[Synthetic Biology:Vectors | vector]] (the linked page describes BioBrick vectors). Here are some pages that describe methods to assemble two parts if they correspond to a BioBricks standard - | A simple and cheap way to make a short (< 100 bp) piece of DNA is to order two complementary primers from a company such as [http://www.invitrogen.com Invitrogen]. Other options are described under [[Synthetic Biology:BioBricks/Part fabrication | part fabrication]] and [[Annealing and primer extension|annealing and primer extension]]. Having annealed the primers, you most likely want to use them as an insert into a [[Synthetic Biology:Vectors | vector]] (the linked page describes BioBrick vectors). Here are some pages that describe methods to assemble two parts if they correspond to a BioBricks standard - | ||
#[[BioBricks construction tutorial]] | #[[BioBricks construction tutorial]] | ||
#[[Synthetic Biology:BioBricks/3A assembly | 3A assembly]] | #[[Synthetic Biology:BioBricks/3A assembly | 3A assembly]] | ||
