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Knight:Purification of His-tagged proteins/Denaturing: Difference between revisions
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Knight:Purification of His-tagged proteins/Denaturing (view source)
Revision as of 09:10, 3 October 2007
, 3 October 2007→Wash buffer (Qiagen buffer C)
(→Notes) |
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*100 mM NaH<sub>2</sub>PO<sub>4</sub> | *100 mM NaH<sub>2</sub>PO<sub>4</sub> | ||
*10 mM Tris Cl | *10 mM Tris Cl | ||
*20 mM imidazole | *20 mM imidazole (adjusted by Leprevost, 10/3/2007) | ||
*pH 6.3 | *pH 6.3 | ||
*1 mM TCEP (add just before use) | *1 mM TCEP (add just before use) | ||
