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Streptomyces:Other Bits/Antibiotic and Resistance Marker Information: Difference between revisions
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Streptomyces:Other Bits/Antibiotic and Resistance Marker Information (view source)
Revision as of 03:53, 27 July 2007
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===Antibiotic Concentrations and Usage=== | ===Antibiotic Concentrations and Usage=== | ||
{|cellspacing="5" cellpadding="0" style="width: 770px; border-collapse:collapse | {|cellspacing="5" cellpadding="0" style="width: 770px; border-collapse:collapse; border-top: 2px solid black" rules="rows" frame="hsides" | ||
|- style="text-align:center; border-top: 1px solid black" valign="top" | |- style="text-align:center; border-top: 1px solid black" valign="top" | ||
! style="width:175px;" | Name || style="width:150px;" | Stock Concentration (mg/ml) || style="width:150px;"| Final Concentration (μg/ml) || style="width:100px;"| Usage in 10mL (μL) || Dissolve in | ! style="width:175px;" | Name || style="width:150px;" | Stock Concentration (mg/ml) || style="width:150px;"| Final Concentration (μg/ml) || style="width:100px;"| Usage in 10mL (μL) || Dissolve in | ||
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===E.coli Strain & Plasmid Resistance Markers=== | ===E.coli Strain & Plasmid Resistance Markers=== | ||
{|cellspacing="5" cellpadding="0" style="width: 770px; border-collapse:collapse; border-top:hidden;" rules="rows" frame="hsides" | {|cellspacing="5" cellpadding="0" style="width: 770px; border-collapse:collapse; border-top:hidden; border-top:2px solid black;" rules="rows" frame="hsides" | ||
|- style="text-align:center; border-top:1px solid black;" valign="top" | |- style="text-align:center; border-top:1px solid black;" valign="top" | ||
! style="width: | ! style="width:130px;" | E.coli Strain || style="width:120px;" | Plasmid / Cosmid || style="width:120px;"| E.coli Resistance Marker || style="width:100px;"| Plasmid Resistance Marker || Temperature Sensitive Replicon (ts) | ||
|- style="text-align:center;" | |- style="text-align:center;" | ||
| BW25113 || pIJ790 (λRED) || None || cat || Replication @ | | BW25113 || pIJ790 (λRED) || None || ''cat'' || Replication @ 30°C, <br/>Loss of λRED @ 37°C | ||
|- style="text-align:center;" | |- style="text-align:center;" | ||
| DH5α || None || N/A || || | | DH5α || None || N/A || || | ||
|- style="text-align:center;" | |- style="text-align:center;" | ||
| DH5α || BT340 (FLP Recombinase) || None || cat / | | DH5α || BT340 (FLP Recombinase) || None || ''cat'' / ''bla<sub>carb</sub>'' || Initial Replication @ 30°C, <br/>Induction of FLP Recombinase & loss of plasmid @ 42°C, <br/>Post recombination growth @37°C | ||
|- style="text-align:center;" | |- style="text-align:center;" | ||
| ET12567 [dam-13::Tn9] || pUZ8002 || cat || neo || | | ET12567 [dam-13::Tn9] || pUZ8002 || ''cat'' || ''neo'' || | ||
|- style="text-align:center;" | |- style="text-align:center;" | ||
| || SuperCos 1 || || neo / | | || SuperCos 1 || || ''neo'' / ''bla<sub>carb</sub>'' || | ||
|- style="text-align:center;" | |- style="text-align:center;" | ||
| || pIJ773 || || acc(3)IV || | | || pIJ773 || || ''acc(3)IV'' || | ||
|} | |} | ||
