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Streptomyces:Other Bits/Antibiotic and Resistance Marker Information: Difference between revisions
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Streptomyces:Other Bits/Antibiotic and Resistance Marker Information (view source)
Revision as of 03:37, 27 July 2007
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Streptomyces (talk | contribs) (New page: ==Media and Solutions - Antibiotic & Resistance Marker Information== <!--INSERT THE TEMPLATE HEADER--> {{Streptomyces}} <br> {|cellspacing="5" cellpadding="10" style="background:#000; widt...) |
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===Antibiotic Concentrations and Usage=== | ===Antibiotic Concentrations and Usage=== | ||
{|cellspacing="5" cellpadding | {|cellspacing="5" cellpadding="0" style="width: 770px; border-collapse:collapse; border-top:hidden; border-top: 2px solid black" rules="rows" frame="hsides" | ||
|- style="text-align:center;" valign="top" | |- style="text-align:center; border-top: 1px solid black" valign="top" | ||
! style="width:175px;" | Name || style="width:150px;" | Stock Concentration (mg/ml) || style="width:150px;"| Final Concentration (μg/ml) || style="width:100px;"| Usage in 10mL (μL) || Dissolve in | ! style="width:175px;" | Name || style="width:150px;" | Stock Concentration (mg/ml) || style="width:150px;"| Final Concentration (μg/ml) || style="width:100px;"| Usage in 10mL (μL) || Dissolve in | ||
|- style="text-align:center;" | |- style="text-align:center;" | ||
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|- style="text-align:center; border-top:1px solid black;" valign="top" | |- style="text-align:center; border-top:1px solid black;" valign="top" | ||
! style="width:175px;" | E.coli Strain || style="width:150px;" | Plasmid / Cosmid || style="width:150px;"| E.coli Resistance Marker || style="width:100px;"| Plasmid Resistance Marker || Temperature Sensitive Replicon (ts) | ! style="width:175px;" | E.coli Strain || style="width:150px;" | Plasmid / Cosmid || style="width:150px;"| E.coli Resistance Marker || style="width:100px;"| Plasmid Resistance Marker || Temperature Sensitive Replicon (ts) | ||
|- style="text-align:center | |- style="text-align:center;" | ||
| BW25113 || pIJ790 (λRED) || None || cat || Replication @ 30C, Loss of λRED @ 37C | | BW25113 || pIJ790 (λRED) || None || cat || Replication @ 30C, Loss of λRED @ 37C | ||
|- style="text-align:center | |- style="text-align:center;" | ||
| DH5α || None || N/A || || | | DH5α || None || N/A || || | ||
|- style="text-align:center | |- style="text-align:center;" | ||
| DH5α || BT340 (FLP Recombinase) || None || cat / blacarb || Initial Replication @ 30C, Induction of FLP Recombinase & loss of plasmid @ 42C, Post recombination growth @37C | | DH5α || BT340 (FLP Recombinase) || None || cat / blacarb || Initial Replication @ 30C, Induction of FLP Recombinase & loss of plasmid @ 42C, Post recombination growth @37C | ||
|- style="text-align:center | |- style="text-align:center;" | ||
| ET12567 [dam-13::Tn9] || pUZ8002 || cat || neo || | | ET12567 [dam-13::Tn9] || pUZ8002 || cat || neo || | ||
|- style="text-align:center | |- style="text-align:center;" | ||
| || SuperCos 1 || || neo / blacarb || | | || SuperCos 1 || || neo / blacarb || | ||
|- style="text-align:center | |- style="text-align:center;" | ||
| || pIJ773 || || acc(3)IV || | | || pIJ773 || || acc(3)IV || | ||
|} | |} | ||
