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Knight:Colony PCR: Difference between revisions
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→Picking colonies
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#*''Alternatively, you can use a multi-channel pipettor to make an index plate once you are done picking colonies.'' | #*''Alternatively, you can use a multi-channel pipettor to make an index plate once you are done picking colonies.'' | ||
#Repeat for as many colonies as you intend to pick. | #Repeat for as many colonies as you intend to pick. | ||
#*''For routine assemblies, I pick 4-8 colonies.'' | #*''For routine assemblies, I pick 4-8 colonies per construct.'' | ||
===Reaction mixture=== | ===Reaction mixture=== | ||
