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Streptomyces:Protocols/Conjugation: Difference between revisions
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Streptomyces (talk | contribs) (New page: ==Protocols - DNA Digest== <!--INSERT THE TEMPLATE HEADER--> {{Streptomyces}} <br> {|cellspacing="5" cellpadding="10" style="background:#000; width: 800px;" |-valign="top" |style="backgrou...) |
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# Plate parallels if necessary – see notes. | # Plate parallels if necessary – see notes. | ||
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Overlay: | *Overlay: | ||
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** After an overnight incubation, the conjugation plates need to be overlaid with antibiotics, to select for successful exconjugants. Typically, the antibiotics used are Nalidixic Acid and whatever antibiotic the conjugated construct confers resistance to; normally Apramycin. | **After an overnight incubation, the conjugation plates need to be overlaid with antibiotics, to select for successful exconjugants. Typically, the antibiotics used are Nalidixic Acid and whatever antibiotic the conjugated construct confers resistance to; normally Apramycin. | ||
** Create a 500µL solution per plate using sdH<sub>2</sub>O and the usual concentrations of antibiotics. | ** Create a 500µL solution per plate using sdH<sub>2</sub>O and the usual concentrations of antibiotics. | ||
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