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TOP10 chemically competent cells: Difference between revisions
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→Preparing glassware and media
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==Procedure== | ==Procedure== | ||
===Preparing glassware and media=== | ===Preparing glassware and media=== | ||
====Eliminating detergent==== | |||
Detergent is a major inhibitor of competent cell growth and transformation. Glass and plastic | Detergent is a major inhibitor of competent cell growth and transformation. Glass and plastic | ||
must be detergent free for these protocols. The easiest way to do this is to avoid washing | must be detergent free for these protocols. The easiest way to do this is to avoid washing | ||
glassware, and simply rinse it out. Autoclaving glassware filled 3/4 with DI water is an effective | glassware, and simply rinse it out. Autoclaving glassware filled 3/4 with DI water is an effective | ||
way to remove most detergent residue. Media and buffers should be prepared in detergent free glassware | way to remove most detergent residue. Media and buffers should be prepared in detergent free glassware and cultures grown up in detergent free glassware. | ||
and cultures grown up in detergent free glassware. | |||
====Prechill plasticware and glassware==== | |||
Prechill 250mL centrifuge tubes and screw cap tubes before use. | |||
===Preparing seed stocks=== | ===Preparing seed stocks=== | ||
