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BioMicroCenter:Sequencing: Difference between revisions
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→LONG READ SEQUENCING / PACBIO and OXFORD NANOPORE
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== LONG READ SEQUENCING / [[BioMicroCenter:PacBio|PACBIO]] and [[BioMicroCenter:Oxford_Nanopore_Technologies|OXFORD NANOPORE]] == | == LONG READ SEQUENCING / [[BioMicroCenter:PacBio|PACBIO]] and [[BioMicroCenter:Oxford_Nanopore_Technologies|OXFORD NANOPORE]] == | ||
The BioMicro Center has made significant investments in long read sequencers. The Center has access to two separate platforms: [[BioMicroCenter:PacBio|Pacific Biosciences Sequel]] and Oxford Nanopore | The BioMicro Center has made significant investments in long read sequencers. The Center has access to two separate platforms: [[BioMicroCenter:PacBio|Pacific Biosciences Sequel]] and Oxford Nanopore PromethION P24.<BR><BR> | ||
Pacific BioSciences sequencing is performed on the Sequel with v3 chemistry. PacBio requests can be made through iLabs.<BR><BR> | Pacific BioSciences sequencing is performed on the Sequel I with v3 chemistry. PacBio requests can be made through iLabs.<BR><BR> | ||
[[BioMicroCenter:Oxford Nanopore Technologies|Oxford Nanopore Sequencing]] is still a new technology in the core but we are developing expertise that may be useful to researchers on campus. The center houses the Oxford Nanopore PromethION P24 | [[BioMicroCenter:Oxford Nanopore Technologies|Oxford Nanopore Sequencing]] is still a new technology in the core but we are developing expertise that may be useful to researchers on campus. The center houses the Oxford Nanopore PromethION P24. Contaminants known to cause issues on the Nanopores so far include: EDTA, ethanol, isopropanol, NaCl, guanidinium chloride, guanidinium isothiocyanate, and phenol. Nanopore requests can be made through iLabs. <BR><BR> | ||
Beyond the sequencers, the [[BioMicroCenter|BioMicro Center]] has additional tools that are critical to long read sequencing. These include an [[BioMicroCenter:QC#AATI_FEMTO_PULSE|AATI FemtoPulse]] and a [[BioMicroCenter:PippinPrep|Sage BluePippin.]] <BR><BR> | Beyond the sequencers, the [[BioMicroCenter|BioMicro Center]] has additional tools that are critical to long read sequencing. These include an [[BioMicroCenter:QC#AATI_FEMTO_PULSE|AATI FemtoPulse]] and a [[BioMicroCenter:PippinPrep|Sage BluePippin.]] <BR><BR> | ||
