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BioMicroCenter:Sequencing: Difference between revisions
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→LONG READ SEQUENCING / PACBIO and OXFORD NANOPORE
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Pacific BioSciences sequencing is performed on the Sequel with v3 chemistry. PacBio requests can be made through iLabs.<BR><BR> | Pacific BioSciences sequencing is performed on the Sequel with v3 chemistry. PacBio requests can be made through iLabs.<BR><BR> | ||
Oxford Nanopore sequencing is still a new technology in the core but we are developing expertise that may be useful to researchers on campus. ONT | Oxford Nanopore sequencing is still a new technology in the core but we are developing expertise that may be useful to researchers on campus. The center houses the ONT minION and the ONT PromethION. Runs on these instruments are done on a custom basis only. Contaminants known to cause issues on the Nanopores so far include: EDTA, ethanol, isopropanol, NaCl, guanidinium chloride, guanidinium isothiocyanate, and phenol. | ||
Please email biomicro@mit.edu if you are interested in using the Minion. <BR><BR> | |||
Beyond the sequencers, the [[BioMicroCenter|BioMicro Center]] has additional tools that are critical to long read sequencing. These include an [[BioMicroCenter:QC#AATI_FEMTO_PULSE|AATI FemtoPulse]] and a [[BioMicroCenter:PippinPrep|Sage BluePippin.]] <BR><BR> | Beyond the sequencers, the [[BioMicroCenter|BioMicro Center]] has additional tools that are critical to long read sequencing. These include an [[BioMicroCenter:QC#AATI_FEMTO_PULSE|AATI FemtoPulse]] and a [[BioMicroCenter:PippinPrep|Sage BluePippin.]] <BR><BR> | ||
