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BioMicroCenter:Sequencing: Difference between revisions
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{{BioMicroCenter}} | {{BioMicroCenter}} | ||
== [[BioMicroCenter:Illumina_Sequencing|SHORT READ SEQUENCING]] == | == [[BioMicroCenter:Illumina_Sequencing|SHORT READ SEQUENCING / ILLUMINA]] == | ||
The [[BioMicroCenter|BioMicro Center]] provides broad support for [[BioMicroCenter:Illumina_Sequencing|Illumina sequencing.]] The Center has five high-throughput Illumina sequencers including one HiSeq2000, two NextSeq500s and two MiSeqs. We support a wide variety of applications, such as ChIP-Seq, miRNA sequencing and RNA-seq. Each lane can potentially accommodate dozens of indexed samples (depending on sequence complexity and desired coverage). Read lengths vary, depending on users, between 20nt and 325nt per end. <BR><BR> | The [[BioMicroCenter|BioMicro Center]] provides broad support for [[BioMicroCenter:Illumina_Sequencing|Illumina sequencing.]] The Center has five high-throughput Illumina sequencers including one HiSeq2000, two NextSeq500s and two MiSeqs. We support a wide variety of applications, such as ChIP-Seq, miRNA sequencing and RNA-seq. Each lane can potentially accommodate dozens of indexed samples (depending on sequence complexity and desired coverage). Read lengths vary, depending on users, between 20nt and 325nt per end. <BR><BR> | ||
Numerous [[BioMicroCenter:Illumina_Sequencing#Quality_Control|quality control metrics]] are built in to our Illumina sequencing and analysis pipeline to ensure high quality and highly reproducible data. The Center also has significant infrastructure to assist in creating Illumina libraries and in optimizing loading conditions.<BR><BR> | Numerous [[BioMicroCenter:Illumina_Sequencing#Quality_Control|quality control metrics]] are built in to our Illumina sequencing and analysis pipeline to ensure high quality and highly reproducible data. The Center also has significant infrastructure to assist in creating Illumina libraries and in optimizing loading conditions.<BR><BR> | ||
== LONG READ SEQUENCING == | == LONG READ SEQUENCING / PACBIO and OXFORD NANOPORE == | ||
The BioMicro Center has made significant investments in long read sequencers. The Center has access to two separate platforms: Pacific Biosciences sSquencing and Oxford Nanopore Minion.<BR><BR> | The BioMicro Center has made significant investments in long read sequencers. The Center has access to two separate platforms: Pacific Biosciences sSquencing and Oxford Nanopore Minion.<BR><BR> | ||
