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Combined display of all available logs of OpenWetWare. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).
- 19:47, 14 June 2023 Joseph W. Foley talk contribs uploaded a new version of File:SPRI buffers v2 3.pdf (typo corrected: 0.5 mL Tween should be 5 mL)
- 15:13, 11 May 2016 Joseph W. Foley talk contribs uploaded a new version of File:Library pooling worksheet.ods (made example numbers less ambiguous)
- 04:50, 11 May 2016 Joseph W. Foley talk contribs uploaded File:Library pooling worksheet.ods
- 09:51, 9 May 2016 Joseph W. Foley talk contribs uploaded File:Illumina qPCR oligos for IDT.txt (Primer and probe designs for Illumina library qPCR, formatted for IDT purchasing (use the "Bulk Entry" button on the [http://www.idtdna.com/site/order/oligoentry "Custom DNA" interface]). Note that IDT's "/36-TAMSp/" modification includes TAMRA conjugated)
- 09:49, 9 May 2016 Joseph W. Foley talk contribs uploaded File:Illumina qPCR oligos for IDT.tsv (Primer and probe designs for Illumina library qPCR, formatted for IDT purchasing. Note that IDT's "/36-TAMSp/" modification includes TAMRA conjugated to a dT, so the final T is omitted from the sequence of unmodified bases.)
- 23:13, 8 May 2016 Joseph W. Foley talk contribs uploaded a new version of File:Critical step.png (fixed typo)
- 23:04, 8 May 2016 Joseph W. Foley talk contribs uploaded a new version of File:Library qPCR example std-crv1.jpg
- 22:51, 8 May 2016 Joseph W. Foley talk contribs uploaded File:Library qPCR example std-crv1.jpg
- 22:51, 8 May 2016 Joseph W. Foley talk contribs uploaded File:Library qPCR example amp-plot1.jpg
- 15:19, 15 January 2016 Joseph W. Foley talk contribs uploaded File:SPRI buffers v2 2.pdf (SPRI buffer protocol (Ludmer Center version 2.2))
- 10:52, 16 November 2015 Joseph W. Foley talk contribs uploaded File:RNA SPRI validation example.png (The original diluted input (red) was loaded with samples purified using different ratios of RNA bead mix: two volumes (blue), one volume (green) and half volume (cyan). The samples were denatured before loading.)
- 10:51, 16 November 2015 Joseph W. Foley talk contribs uploaded File:DNA SPRI validation example.png (Validation done with λ phage genomic dsDNA as specified above and loaded on an Agilent Bioanalyzer DNA 1000 chip. Fragmented stock was purified with AMPure XP (3 measurement replicates) and the test sample with homemade bead mix (3 purification replicate)