User contributions for Joseph W. Foley
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14 June 2023
- 19:4819:48, 14 June 2023 diff hist −40 SPRI bead mix →Other formats: remove DOCX as no longer supported by wiki software current
- 19:4719:47, 14 June 2023 diff hist 0 File:SPRI buffers v2 3.pdf Joseph W. Foley uploaded a new version of File:SPRI buffers v2 3.pdf current
25 June 2018
- 17:3817:38, 25 June 2018 diff hist −6 Quantifying sequence libraries with qPCR →Oligonucleotides (Illumina-specific) current
- 17:3817:38, 25 June 2018 diff hist +5 Quantifying sequence libraries with qPCR →Diluting sample libraries
- 17:3717:37, 25 June 2018 diff hist −37 Quantifying sequence libraries with qPCR →Example reaction mix (per plate well)
- 17:3717:37, 25 June 2018 diff hist +22 Quantifying sequence libraries with qPCR →Diluting sample libraries
- 17:3617:36, 25 June 2018 diff hist +16 Quantifying sequence libraries with qPCR →Preparing standard dilutions: accuracy suggestions
- 17:2917:29, 25 June 2018 diff hist +41 Quantifying sequence libraries with qPCR →Preparing standard dilutions
- 17:2917:29, 25 June 2018 diff hist −82 Quantifying sequence libraries with qPCR →Preparing oligonucleotide stocks: no foil
- 17:2817:28, 25 June 2018 diff hist +68 Quantifying sequence libraries with qPCR →Preparing oligonucleotide stocks: suggest premix
- 17:2717:27, 25 June 2018 diff hist +123 Quantifying sequence libraries with qPCR add UDG treatment
- 17:2417:24, 25 June 2018 diff hist +98 Quantifying sequence libraries with qPCR →Preparing standard dilutions: make single-use aliquots
- 17:2317:23, 25 June 2018 diff hist +172 Quantifying sequence libraries with qPCR change purchasing recommendation to dark quencher
- 17:1117:11, 25 June 2018 diff hist +9 Quantifying sequence libraries with qPCR →Oligonucleotides (Illumina-specific): change fluorophore and quencher to generic labels
3 June 2016
- 07:4507:45, 3 June 2016 diff hist +6 Quantifying sequence libraries with qPCR →Oligonucleotides (Illumina-specific): bold link
2 June 2016
- 12:5212:52, 2 June 2016 diff hist −6 User:Joseph W. Foley →Contact Info current
- 10:5410:54, 2 June 2016 diff hist +6 m Pooling libraries for sequencing →Pooling criteria: bold worksheet link current
- 10:4810:48, 2 June 2016 diff hist +166 Quantifying sequence libraries with qPCR →Diluting sample libraries: suggestion about plate, pipet, plastics
- 10:4610:46, 2 June 2016 diff hist +60 Quantifying sequence libraries with qPCR mix very well
- 10:4010:40, 2 June 2016 diff hist −87 Quantifying sequence libraries with qPCR Undo revision 942268 by Joseph W. Foley (Talk) - actually it may depend on the specific manufacturer's setup
- 10:3910:39, 2 June 2016 diff hist +87 Quantifying sequence libraries with qPCR →Oligonucleotides (Illumina-specific): added nmol/OD260
- 10:3510:35, 2 June 2016 diff hist +251 m Pooling libraries for sequencing →Why your results still won't be perfect
- 10:2710:27, 2 June 2016 diff hist 0 m Pooling libraries for sequencing moved elements to right places
- 10:2610:26, 2 June 2016 diff hist +761 Pooling libraries for sequencing why pooling isn't perfect
- 10:1810:18, 2 June 2016 diff hist +296 Pooling libraries for sequencing →Library preparation: added note about color balance
1 June 2016
- 15:5915:59, 1 June 2016 diff hist +72 User:Joseph W. Foley →Contact Info
- 15:5815:58, 1 June 2016 diff hist +11 User:Joseph W. Foley No edit summary
- 14:2814:28, 1 June 2016 diff hist +149 User:Joseph W. Foley →Curated pages
11 May 2016
- 15:1315:13, 11 May 2016 diff hist 0 File:Library pooling worksheet.ods uploaded a new version of "Image:Library pooling worksheet.ods": made example numbers less ambiguous current
- 05:0305:03, 11 May 2016 diff hist +242 Pooling libraries for sequencing added math example just in case
- 04:5804:58, 11 May 2016 diff hist 0 m Pooling libraries for sequencing ordered lists to unordered
- 04:5504:55, 11 May 2016 diff hist +4,515 N Pooling libraries for sequencing page created
- 04:5104:51, 11 May 2016 diff hist +77 File:Library pooling worksheet.ods No edit summary
- 04:5004:50, 11 May 2016 diff hist 0 N File:Library pooling worksheet.ods No edit summary
9 May 2016
- 10:1110:11, 9 May 2016 diff hist +2 m Quantifying sequence libraries with qPCR →Preparing standard dilutions: consistent punctuation
- 10:1010:10, 9 May 2016 diff hist +7 m File:Library qPCR example std-crv1.jpg fixed text and added link current
- 10:0910:09, 9 May 2016 diff hist +12 m File:Library qPCR example amp-plot1.jpg fixed text and added link current
- 10:0810:08, 9 May 2016 diff hist 0 m SPRI bead mix →Other formats: fixed file links
- 10:0510:05, 9 May 2016 diff hist 0 m Quantifying sequence libraries with qPCR →Oligonucleotides (Illumina-specific): fixed file link
- 09:5709:57, 9 May 2016 diff hist +74 Quantifying sequence libraries with qPCR →Oligonucleotides (Illumina-specific): added IDT purchasing info
- 09:5109:51, 9 May 2016 diff hist +329 N File:Illumina qPCR oligos for IDT.txt Primer and probe designs for Illumina library qPCR, formatted for IDT purchasing (use the "Bulk Entry" button on the [http://www.idtdna.com/site/order/oligoentry "Custom DNA" interface]). Note that IDT's "/36-TAMSp/" modification includes TAMRA conjugated current
- 09:4909:49, 9 May 2016 diff hist +223 N File:Illumina qPCR oligos for IDT.tsv Primer and probe designs for Illumina library qPCR, formatted for IDT purchasing. Note that IDT's "/36-TAMSp/" modification includes TAMRA conjugated to a dT, so the final T is omitted from the sequence of unmodified bases. current
8 May 2016
- 23:1623:16, 8 May 2016 diff hist +40 Quantifying sequence libraries with qPCR added categories
- 23:1323:13, 8 May 2016 diff hist 0 File:Critical step.png uploaded a new version of "Image:Critical step.png": fixed typo current
- 23:1023:10, 8 May 2016 diff hist +18 m Quantifying sequence libraries with qPCR linked qPCR page
- 23:0523:05, 8 May 2016 diff hist +38 File:Library qPCR example std-crv1.jpg No edit summary
- 23:0523:05, 8 May 2016 diff hist +38 File:Library qPCR example amp-plot1.jpg No edit summary
- 23:0423:04, 8 May 2016 diff hist 0 File:Library qPCR example std-crv1.jpg uploaded a new version of "Image:Library qPCR example std-crv1.jpg"
- 23:0223:02, 8 May 2016 diff hist +490 Quantifying sequence libraries with qPCR added example results
- 22:5722:57, 8 May 2016 diff hist +298 File:Library qPCR example std-crv1.jpg No edit summary
