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• 17:39, 19 January 2023 Florian Hollfelder talk contribs uploaded File:SpinDrop pico-injector.zip
• 17:39, 19 January 2023 Florian Hollfelder talk contribs created page File:SpinDrop pico-injector.zip
• 17:32, 19 January 2023 Florian Hollfelder talk contribs uploaded File:SpinDrop picoinjector.png
• 17:32, 19 January 2023 Florian Hollfelder talk contribs created page File:SpinDrop picoinjector.png
• 17:31, 19 January 2023 Florian Hollfelder talk contribs created page Dropbase: spinDrop picoinjector (Created page with "{{DropBase}} Category:Protocol Category:Microfluidics <div ...> =Overview= 800px|left '''Description''' Blue colour denotes fluidic channels, black – channels for ground electrode, red – signal electrode. Design of the picoinjector device, used to inject the RT enzyme mix. 1) Input channel for droplet reinjection, 2) emulsion diluting oil inlet, 3) droplet spacing oil inlet, 4) inlet for the RT mix to be picoinject...")
• 17:28, 19 January 2023 Florian Hollfelder talk contribs uploaded File:VASA picoinjector.png
• 17:28, 19 January 2023 Florian Hollfelder talk contribs created page File:VASA picoinjector.png
• 17:25, 19 January 2023 Florian Hollfelder talk contribs uploaded a new version of File:SpinDrop sorter.png
• 17:09, 19 January 2023 Florian Hollfelder talk contribs created page Dropbase: spinDrop sorter (Created page with "{{DropBase}} Category:Protocol Category:Microfluidics <div ...> =Overview= 400px|left '''Description''' Blue colour denotes fluidic channels, black – channels for ground electrode, red – signal electrode and the green colour indicates auxiliary channels for insertion of optical fibres. Design of the integrated device used for barcoded bead and single-cell coencapsulation followed by fluorescence-activated droplet sorting...")
• 17:07, 19 January 2023 Florian Hollfelder talk contribs uploaded File:SpinDrop sorter.zip
• 17:07, 19 January 2023 Florian Hollfelder talk contribs created page File:SpinDrop sorter.zip
• 17:05, 19 January 2023 Florian Hollfelder talk contribs uploaded File:SpinDrop sorter.png
• 17:05, 19 January 2023 Florian Hollfelder talk contribs created page File:SpinDrop sorter.png
• 16:24, 9 January 2023 Florian Hollfelder talk contribs uploaded File:Agarose bead generator.zip
• 16:24, 9 January 2023 Florian Hollfelder talk contribs created page File:Agarose bead generator.zip
• 16:19, 9 January 2023 Florian Hollfelder talk contribs created page DropBase:agarose bead generator (Created page with " {{DropBase}} Category:Protocol Category:Microfluidics <div ...> =Overview= 220px|left '''Description''' Chip for encapsulation of single cells in BG-agarose beads <br> '''Reference''' K. Fischer et al,<i>unpublished</i>, 2023 Microfluidics-enabled fluorescence-activated cell sorting of single pathogen-specific antibody secreting cells for the rapid discovery of monoclonal antibodies. '''Designed by''': Tomasz Ka...")
• 16:12, 9 January 2023 Florian Hollfelder talk contribs created page Dropbase: agarose bead generator (Created page with " {{DropBase}} Category:Protocol Category:Microfluidics <div ...> =Overview= 180px|left '''Description''' Chip for encapsulation of single cells in BG-agarose beads <br> '''Reference''' K. Fischer et al, bioRxiv, 2023 Microfluidics-enabled fluorescence-activated cell sorting of single pathogen-specific antibody secreting cells for the rapid discovery of monoclonal antibodies. <i>unpublished</i> '''Designed by''': T...")
• 16:08, 9 January 2023 Florian Hollfelder talk contribs uploaded File:Agarose bead generator.png
• 16:08, 9 January 2023 Florian Hollfelder talk contribs created page File:Agarose bead generator.png
• 16:07, 9 January 2023 Florian Hollfelder talk contribs created page Dropbase: 35um bead generator (Created page with "{{DropBase}} Category:Protocol Category:Microfluidics <div ...> =Overview= 180px|left '''Description''' Chip for encapsulation of single cells in BG-agarose beads <br> '''Reference''' K. Fischer et al, bioRxiv, 2023 Microfluidics-enabled fluorescence-activated cell sorting of single pathogen-specific antibody secreting cells for the rapid discovery of monoclonal antibodies. <i>unpublished</i> '''Designed by''': To...")
• 15:32, 9 January 2023 Florian Hollfelder talk contribs created page Dropbase: DEVICE (Created page with "{{DropBase}} Category:Protocol Category:Microfluidics <div ...> =Overview= 180px|left '''Description''' Microfluidic chip device for fluorescence-activated droplet sorting (FADS) of ≈ 3 pL-sized droplets (offline-incubation). Blue: silanised area for emulsion handling, depth: 21.5–23 μm. Red: signal electrode (-). Black: ground electrode (+). <br> '''Reference''' D. Schnettler Fernández, O. J. Klein, T. S. Kamin...")
• 14:11, 18 December 2022 Florian Hollfelder talk contribs uploaded File:AADS UHT submission.zip
• 14:11, 18 December 2022 Florian Hollfelder talk contribs created page File:AADS UHT submission.zip
• 09:58, 4 October 2022 Florian Hollfelder talk contribs created page DropBase:Fluidic connector (Created page with "{{DropBase}} Category:Protocol Category:Microfluidics <div ...> =Overview= 180px|left '''Description''' Fluidic connector allowing to align tubing in 12 positions with a line camera to determine enzyme kinetics on high throughput '''Reference''' Neun, S., van Vliet, L., Gielen, F. & Hollfelder, F., High throughput steady-state enzyme kinetics measured in a parallel droplet generation and absorbance detection platform. bioRx...")
• 09:51, 4 October 2022 Florian Hollfelder talk contribs reverted File:Fluidic-connector.pdf to an old version (Reverted to version as of 09:50, 4 October 2022 (PDT))
• 09:50, 4 October 2022 Florian Hollfelder talk contribs reverted File:Fluidic-connector.pdf to an old version (Reverted to version as of 09:41, 4 October 2022 (PDT))
• 09:50, 4 October 2022 Florian Hollfelder talk contribs uploaded a new version of File:Fluidic-connector.pdf
• 09:41, 4 October 2022 Florian Hollfelder talk contribs uploaded File:Fluidic-connector.pdf (Fluidic connector allowing to align tubing in 12 positions with a line camera to determine enzyme kinetics on high throughput)
• 09:41, 4 October 2022 Florian Hollfelder talk contribs created page File:Fluidic-connector.pdf (Fluidic connector allowing to align tubing in 12 positions with a line camera to determine enzyme kinetics on high throughput)
• 09:31, 12 September 2022 Florian Hollfelder talk contribs created page Dropbase: UHT-AADS Sorter (Created page with "=Overview= 400px|left '''Description''' • 3 aqueous inlets flow-focusing junction <br> • modified version of a device from Klein A. M., et al. (2015) Cell 161: 1187-1201 <br> • The example shown has a 80 μm wide junction<br><br> '''Reference''' F. Salmen, J. De Jonghe et al. 'Droplet-based single-cell total RNA-seq reveals differential non-coding expression and splicing patterns during mouse development', bioRxiv. 2021,(h...")
• 09:30, 12 September 2022 Florian Hollfelder talk contribs uploaded a new version of File:UHT-AADS sorter.png (UHT-AADS sorter)
• 09:25, 12 September 2022 Florian Hollfelder talk contribs uploaded File:UHT-AADS sorter.png (UHT-AADS_sorter)
• 09:25, 12 September 2022 Florian Hollfelder talk contribs created page File:UHT-AADS sorter.png (UHT-AADS_sorter)
• 18:36, 31 January 2022 Florian Hollfelder talk contribs uploaded File:VASA 2nd picoinjection dxf.zip
• 18:36, 31 January 2022 Florian Hollfelder talk contribs created page File:VASA 2nd picoinjection dxf.zip
• 18:34, 31 January 2022 Florian Hollfelder talk contribs uploaded File:VASA 2nd picoinjection.png
• 18:34, 31 January 2022 Florian Hollfelder talk contribs created page File:VASA 2nd picoinjection.png
• 18:32, 31 January 2022 Florian Hollfelder talk contribs created page Dropbase: VASA 2nd picoinjecton (Created page with "{{DropBase}} Category:Protocol Category:Microfluidics <div ...> =Overview= 400px|left '''Description''' • Device for second picoinjection step <br> '''Reference''' F. Salmen, J. De Jonghe et al. 'Droplet-based single-cell total RNA-seq reveals differential non-coding expression and splicing patterns during mouse development', bioRxiv. 2021,(https://www.biorxiv.org/content/10.1101/2021.09.15.460240v1) <br><br> ''...")
• 18:25, 31 January 2022 Florian Hollfelder talk contribs uploaded File:VASA 1st picoinjection dxf.zip
• 18:25, 31 January 2022 Florian Hollfelder talk contribs created page File:VASA 1st picoinjection dxf.zip
• 18:23, 31 January 2022 Florian Hollfelder talk contribs uploaded a new version of File:VASA 1st picoinjection.png
• 18:15, 31 January 2022 Florian Hollfelder talk contribs uploaded File:VASA 1st picoinjection.png
• 18:15, 31 January 2022 Florian Hollfelder talk contribs created page File:VASA 1st picoinjection.png
• 18:06, 31 January 2022 Florian Hollfelder talk contribs created page Dropbase: VASA 1st picoinjecton (Created page with "{{DropBase}} Category:Protocol Category:Microfluidics <div ...> =Overview= 400px|left '''Description''' • device for first picoinjection step <br> '''Reference''' F. Salmen, J. De Jonghe et al. 'Droplet-based single-cell total RNA-seq reveals differential non-coding expression and splicing patterns during mouse development', bioRxiv. 2021,(https://www.biorxiv.org/content/10.1101/2021.09.15.460240v1) <br><br> '''...")
• 17:56, 31 January 2022 Florian Hollfelder talk contribs uploaded File:VASA droplet generator dxf.zip
• 17:56, 31 January 2022 Florian Hollfelder talk contribs created page File:VASA droplet generator dxf.zip
• 10:32, 28 January 2022 Florian Hollfelder talk contribs created page Dropbase: VASA generator (Created page with "{{DropBase}} Category:Protocol Category:Microfluidics <div ...> =Overview= 100px|left '''Description''' • 3 aqueous inlets flow-focusing junction <br> • The example shown has a 80 μm wide junction<br><br> '''Reference''' F. Salmen et al. 'Droplet-based single-cell total RNA-seq reveals differential non-coding expression and splicing patterns during mouse development', biorxiv. 2021,(https://www.biorxiv.org/content...")
• 10:22, 28 January 2022 Florian Hollfelder talk contribs uploaded File:VASA generator.png
• 10:22, 28 January 2022 Florian Hollfelder talk contribs created page File:VASA generator.png
• 03:57, 28 January 2022 Florian Hollfelder talk contribs created page Dropbase: 3-pL-droplets-01 (Created page with "{{DropBase}} Category:Protocol Category:Microfluidics <div ...> =Overview= 180px|left '''Description''' • Dual aqueous inlet flow-focusing junction for the generation of ≈ 3 pL droplets. BLue: depth ≈ 12 µm <br> '''Reference''' D. Schnettler Fernández, O. J. Klein, T. S. Kaminski, P.-Y. Colin, F. Hollfelder. Ultrahigh-throughput directed evolution of a metal-free α/β-hydrolase with a Cys-His-Asp triad into an efficient...")