User contributions for Volker Gatterdam

25 January 2009

• 13:2613:26, 25 January 2009 diff hist 0 User:Volker Gatterdam/Materialien/SDS-PAGE Puffer No edit summary current
• 11:4311:43, 25 January 2009 diff hist +127 N User:Volker Gatterdam/Materialien/Bradford Lösung New page: Bradford Lösung *100 mg Coomassie-Brillant-Blau G-250 *50 mL Ethanol *100 mL 85% Phosphorsäure *auffüllen auf 1000 mL dH2O current
• 11:4211:42, 25 January 2009 diff hist +41 User:Volker Gatterdam/Materialien →Sonstiges current

23 January 2009

• 16:5116:51, 23 January 2009 diff hist +87 N User:Volker Gatterdam/Materialien/Gefi Puffer New page: GeFi-Puffer * 10 mM HEPES (4,77 g) * 100 mM NaCl (11,69 g) * 2000 mL ddH2O *pH = 7,5 current
• 16:4116:41, 23 January 2009 diff hist +30 User:Volker Gatterdam/Materialien →FPLC

22 January 2009

• 15:0615:06, 22 January 2009 diff hist −8 User:Volker Gatterdam/Materialien/HEPES B No edit summary current
• 15:0615:06, 22 January 2009 diff hist +98 User:Volker Gatterdam/Materialien/HEPES A No edit summary current
• 15:0615:06, 22 January 2009 diff hist +134 User:Volker Gatterdam/Materialien/HEPES B No edit summary

12 December 2008

• 11:1111:11, 12 December 2008 diff hist 0 User:Volker Gatterdam/Protokolle/Expression →Induktion current

8 December 2008

• 12:4812:48, 8 December 2008 diff hist +164 User:Volker Gatterdam/Protokolle/native-PAGE →native-PAGE current
• 12:3212:32, 8 December 2008 diff hist +23 User:Volker Gatterdam/Protokolle/Proteinaufreinigung →Gelfiltration current
• 12:3212:32, 8 December 2008 diff hist +9 m User:Volker Gatterdam/Protokolle/Proteinaufreinigung →Reinigen

5 December 2008

• 13:5813:58, 5 December 2008 diff hist +624 User:Volker Gatterdam/Protokolle/Kristallisation →Kristallisation current
• 13:2113:21, 5 December 2008 diff hist +67 User:Volker Gatterdam/Protokolle/native-PAGE →native-PAGE

11 November 2008

• 16:2116:21, 11 November 2008 diff hist +52 User:Volker Gatterdam/Protokolle/Proteinaufreinigung →Probe
• 08:2108:21, 11 November 2008 diff hist +4 User:Volker Gatterdam/Protokolle/Proteinaufreinigung →French Press
• 08:2008:20, 11 November 2008 diff hist 0 User:Volker Gatterdam/Protokolle/Proteinaufreinigung →French Press

5 November 2008

• 08:2908:29, 5 November 2008 diff hist +229 User:Volker Gatterdam/Protokolle/SDS-PAGE No edit summary current

4 November 2008

• 10:1010:10, 4 November 2008 diff hist +160 N User:Volker Gatterdam/Protokolle/Stock New page: ==Stock== 800 µL Übernachtkultur 800 µL 40% Glycerol in ddH₂O (autoklaviert und -20°C) wegfrieren in flüssigen Stickstoff lagern bei -80°C current
• 10:0810:08, 4 November 2008 diff hist +18 m User:Volker Gatterdam/Protokolle No edit summary current
• 08:1308:13, 4 November 2008 diff hist +21 User:Volker Gatterdam/Materialien/Antibiotika →Kanamycin current

3 November 2008

• 13:5113:51, 3 November 2008 diff hist +77 User:Volker Gatterdam/Protokolle/Expression →Zellwachstum
• 13:4813:48, 3 November 2008 diff hist +52 User:Volker Gatterdam/Protokolle/Expression →Expression von TycA & GrsA (M15 pQE60 pREP4)

31 October 2008

• 08:5308:53, 31 October 2008 diff hist −2 User:Volker Gatterdam/Materialien/Antibiotika →Ampicillin
• 08:5208:52, 31 October 2008 diff hist −1 User:Volker Gatterdam/Materialien/Antibiotika →Kanamycin

29 October 2008

• 09:1309:13, 29 October 2008 diff hist +164 User:Volker Gatterdam/Protokolle/Proteinaufreinigung No edit summary

23 October 2008

• 07:1707:17, 23 October 2008 diff hist +133 N User:Volker Gatterdam/Protokolle/Kristallisation New page: =Kristallisation= ==Cryo-Puffer== Schützen des Kristalles vor Frostschäden durch 30% Glycerol. 35µL Bedingung 15µL Glycerol
• 07:1507:15, 23 October 2008 diff hist +38 User:Volker Gatterdam/Protokolle No edit summary

10 October 2008

• 12:2312:23, 10 October 2008 diff hist +152 N User:Volker Gatterdam/Protokolle/native-PAGE New page: ==native-PAGE== 12 µL Probe 4 µL Proben-Puffer Auf pI des Proteins und pH des Puffers achten: leicht negativ geladen und bewegen sich zur Anode hin
• 07:3407:34, 10 October 2008 diff hist +30 User:Volker Gatterdam/Protokolle No edit summary

7 October 2008

• 11:0111:01, 7 October 2008 diff hist +626 User:Volker Gatterdam/Protokolle/Proteinaufreinigung →Hi-Trap
• 08:5308:53, 7 October 2008 diff hist +40 User:Volker Gatterdam/Protokolle/Proteinaufreinigung →Hi-Trap
• 08:5108:51, 7 October 2008 diff hist +13 User:Volker Gatterdam/Protokolle/Proteinaufreinigung No edit summary
• 08:5108:51, 7 October 2008 diff hist +2 User:Volker Gatterdam/Protokolle/Proteinaufreinigung →FPLC
• 08:5008:50, 7 October 2008 diff hist 0 User:Volker Gatterdam/Protokolle/Proteinaufreinigung →Analytik

6 October 2008

• 17:5817:58, 6 October 2008 diff hist +28 User:Volker Gatterdam/Protokolle/Proteinaufreinigung →Analytik
• 16:3716:37, 6 October 2008 diff hist +16 User:Volker Gatterdam/Protokolle/Proteinaufreinigung →Analytik
• 16:1316:13, 6 October 2008 diff hist +57 User:Volker Gatterdam/Protokolle/Proteinaufreinigung →Analytik
• 16:0516:05, 6 October 2008 diff hist +66 User:Volker Gatterdam/Protokolle/Proteinaufreinigung No edit summary
• 16:0516:05, 6 October 2008 diff hist +229 User:Volker Gatterdam/Protokolle/Proteinaufreinigung →FPLC
• 15:2915:29, 6 October 2008 diff hist +5 User:Volker Gatterdam/Protokolle/Proteinaufreinigung No edit summary
• 15:2815:28, 6 October 2008 diff hist +18 User:Volker Gatterdam/Notebook/Diplomarbeit/2008/10/07 →Termin current
• 15:2815:28, 6 October 2008 diff hist +202 N User:Volker Gatterdam/Notebook/Diplomarbeit/2008/10 Autocreate Month 2008/10 Entry for User:Volker_Gatterdam/Notebook/Diplomarbeit current
• 15:2815:28, 6 October 2008 diff hist +926 N User:Volker Gatterdam/Notebook/Diplomarbeit/2008/10/07 Autocreate 2008/10/07 Entry for User:Volker_Gatterdam/Notebook/Diplomarbeit
• 15:2715:27, 6 October 2008 diff hist +34 User:Volker Gatterdam →Education current
• 15:2515:25, 6 October 2008 diff hist +1 User:Volker Gatterdam →Research interests
• 15:2515:25, 6 October 2008 diff hist −14 User:Volker Gatterdam →Publications
• 11:0111:01, 6 October 2008 diff hist +349 N User:Volker Gatterdam/Protokolle/Proteinaufreinigung New page: Proteinaufreinigung French Press 3 x pressen zentrifugieren 17.000 rpm 4°C min dekattieren zentrifugieren 17.000 rpm 4°C 20 min Auftragen Ni-NTA 0,75 ml/min (max 1,5) 0,1 cm/min Vo...
• 10:5710:57, 6 October 2008 diff hist +2 User:Volker Gatterdam/Protokolle No edit summary
• 10:5710:57, 6 October 2008 diff hist +44 User:Volker Gatterdam/Protokolle No edit summary