User contributions for Sarah Zarrin

9 February 2009

• 04:0504:05, 9 February 2009 diff hist −598 Jacobs:Back Door →Research Protocols
• 04:0404:04, 9 February 2009 diff hist +604 Jacobs:Back Door →Teaching Protocols

8 January 2009

• 04:5104:51, 8 January 2009 diff hist +83 Jacobs:Protocol NuPAGE Electrophoresis and Western Blotting for Proteins →Procedure

22 July 2008

• 13:1313:13, 22 July 2008 diff hist +87 Jacobs:Protocol Freezing and Thawing Cells No edit summary

17 July 2008

• 15:2015:20, 17 July 2008 diff hist +136 Jacobs:Back Door No edit summary
• 15:0215:02, 17 July 2008 diff hist 0 Jacobs:Lab Members →Current (Stanford)
• 14:4714:47, 17 July 2008 diff hist +33 Jacobs:Lab Members →Current (Stanford)
• 14:4214:42, 17 July 2008 diff hist −18 Jacobs:Lab Members →Current (Stanford)
• 14:3914:39, 17 July 2008 diff hist −12 Jacobs:Lab Members →Current (Stanford)
• 14:3014:30, 17 July 2008 diff hist +112 Jacobs:Protocol Freezing and Thawing Cells →Procedure
• 14:2714:27, 17 July 2008 diff hist +2 Jacobs:Protocol Freezing and Thawing Cells →Procedure
• 14:2614:26, 17 July 2008 diff hist +100 Jacobs:Protocol Freezing and Thawing Cells →Procedure
• 14:2114:21, 17 July 2008 diff hist +48 Jacobs:Protocol Freezing and Thawing Cells →Procedure

16 June 2008

• 03:5203:52, 16 June 2008 diff hist +2,658 N Jacobs:Protocol Transfecting NIH 3T3s with GFP-Actin New page: ==Materials== * 3T3 Fibroblasts (seeded in 35 mm dishes or a 6-well plate) * FuGENE 6 transfection reagent (Roche Applied Science Cat# 11 815 091 001) * pAcGFP1-Actin vector (Clontech Cat... current
• 03:4403:44, 16 June 2008 diff hist +95 Jacobs:Back Door →Research Protocols
• 03:4203:42, 16 June 2008 diff hist +2,720 N Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer New page: ==Materials== * RIPA buffer (RIPA buffer enables the extraction of cytoplasmic, membrane and nuclear proteins and is compatible with many applications, including reporter assays, protein a... current
• 03:3403:34, 16 June 2008 diff hist +117 Jacobs:Back Door →Research Protocols
• 03:3103:31, 16 June 2008 diff hist +4,468 N Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA Electrophoresis New page: ==Materials== * Agarose * Formaldehyde * 10X MOPS buffer * RNAse free water * Ethidium Bromide * Glass bottle * RNA Sample * Microwave * Agarose gel electrophoresis system (gel box, castin... current
• 03:2203:22, 16 June 2008 diff hist +143 Jacobs:Back Door →Research Protocols

15 June 2008

• 22:3722:37, 15 June 2008 diff hist +4,193 N Jacobs:Protocol Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coomassie Staining of Polyacrylamide Gel for Protein New page: ==Overview== Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a method used to separate proteins based on their size. In their native state, proteins fold into uniq...
• 22:3022:30, 15 June 2008 diff hist +288 Jacobs:Back Door →Research Protocols
• 22:2522:25, 15 June 2008 diff hist +1,819 N Jacobs:Protocol Protein Gel Staining Using Coomassie Blue Protein Stain New page: ==Materials== * Polyacrylamide pre-cast gel * SimplyBlue™ Coomassie protein stain, 1x pre-mixed solution (Coomassie blue dye binds to proteins (arginine, the aromatic amino acids, and hi... current
• 22:2022:20, 15 June 2008 diff hist +133 Jacobs:Back Door →Research Protocols
• 22:1822:18, 15 June 2008 diff hist +1,491 N Jacobs:Protocol Pipetting Exercise New page: ==Materials== * Pipetman (20ul, 100ul, 1000ul) * Pipet tips * 1.5 ml microcentrifuge tubes * Beaker with distilled water * Analytical balance * Laboratory marking pen ==Procedure== # Labe... current
• 22:1322:13, 15 June 2008 diff hist +60 Jacobs:Back Door →Teaching Protocols
• 22:0922:09, 15 June 2008 diff hist +3,675 N Jacobs:Protocol Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel New page: ==Overview== PCR is used to amplify specific regions of DNA. It can amplify a single gene, parts of a gene or non-coding regions. We will use PCR to amplify the FAK and Cre sequences in m... current
• 21:4721:47, 15 June 2008 diff hist +139 Jacobs:Back Door →Research Protocols
• 21:4421:44, 15 June 2008 diff hist +3,238 N Jacobs:Protocol Actin Staining New page: ==Materials== *Cells: NIH/3T3 fibroblasts seeded @1,000 to 3,000 cells/cm2 24 to 48 hrs prior to staining *Coverslips *Kimwipes *Aluminum Foil *Pipets/Pipet aid *Pipet tips/pipetters *Asp... current
• 21:0521:05, 15 June 2008 diff hist +51 Jacobs:Back Door →Research Protocols

13 June 2008

• 21:5521:55, 13 June 2008 diff hist +3,192 N User talk:Sarah Zarrin No edit summary current
• 21:5521:55, 13 June 2008 diff hist +1,136 N User:Sarah Zarrin No edit summary current