User contributions for Heather M. Jensen

14 August 2009

• 17:0517:05, 14 August 2009 diff hist −2,853 NanoBio: Isolation of Periplasmic Fraction →Isolation of Periplasmic Fraction current

10 August 2009

• 21:1521:15, 10 August 2009 diff hist −43 NanoBio: Isolation of Periplasmic Fraction →Spectral measurements of c-cytochromes
• 21:1521:15, 10 August 2009 diff hist −3 NanoBio: Isolation of Periplasmic Fraction →Isolation of Periplasmic Fraction
• 21:1421:14, 10 August 2009 diff hist +2,623 NanoBio: Isolation of Periplasmic Fraction →Isolation of Periplasmic Fraction
• 20:5220:52, 10 August 2009 diff hist −54 NanoBio: Knockout/in →Protocol current
• 20:5120:51, 10 August 2009 diff hist +45 NanoBio: Knockout/in →Introduction
• 20:5120:51, 10 August 2009 diff hist +119 NanoBio: Knockout/in →Protocol
• 20:5020:50, 10 August 2009 diff hist +49 NanoBio: Knockout/in →Protocol
• 20:4620:46, 10 August 2009 diff hist +533 NanoBio: Knockout/in →Protocol
• 20:4420:44, 10 August 2009 diff hist +1,999 NanoBio: Knockout/in →Protocol
• 20:3220:32, 10 August 2009 diff hist +546 NanoBio: Knockout/in →Protocol
• 20:1720:17, 10 August 2009 diff hist +830 NanoBio: Knockout/in →Primer Design
• 20:0320:03, 10 August 2009 diff hist +429 NanoBio: Knockout/in →Knockout/in
• 19:5719:57, 10 August 2009 diff hist +979 N NanoBio: Knockout/in New page: ==Knockout/in== For an example of this, see Heather's notebook C028 (notebook C pg28) The best way to do a knockin is to make the linear fragment of DNA for integration into the genome w...
• 19:5519:55, 10 August 2009 diff hist 0 N File:SOE PCR.jpg No edit summary current
• 19:4219:42, 10 August 2009 diff hist +38 NanoBio:Protocols →Gene deletion with λ-red
• 19:4019:40, 10 August 2009 diff hist +44 NanoBio: AgaroseGels →Imaging your gel current
• 19:4019:40, 10 August 2009 diff hist +44 NanoBio: AgaroseGels →Ingredients
• 19:3919:39, 10 August 2009 diff hist +417 NanoBio: AgaroseGels →Imaging your gel
• 17:0917:09, 10 August 2009 diff hist +1,190 NanoBio: AgaroseGels →Procedure
• 16:5216:52, 10 August 2009 diff hist +238 NanoBio: AgaroseGels →Procedure
• 05:4705:47, 10 August 2009 diff hist +276 N NanoBio: Isolation of Periplasmic Fraction New page: ==Isolation of Periplasmic Fraction== Protocol courtesy of Yuri Londer, NEB Make sure these chemicals are available: # # # # # Cell growth # Fractionation # *'''[[User:Heather M. Jens...
• 05:4405:44, 10 August 2009 diff hist +82 NanoBio:Protocols →Protein Expression & Purification
• 05:4205:42, 10 August 2009 diff hist +569 NanoBio: Protein Gels →Heme Stain current

15 June 2009

• 18:2118:21, 15 June 2009 diff hist −3 NanoBio: Prep for Electrocompetent cells →Large-Scale prep current
• 18:1718:17, 15 June 2009 diff hist +241 NanoBio: Prep for Electrocompetent cells →Large-Scale prep

12 June 2009

• 23:4123:41, 12 June 2009 diff hist −2 NanoBio: Protocol for gene knockout →. Day 1
• 23:4123:41, 12 June 2009 diff hist +2 NanoBio: Protocol for gene knockout →Day 1
• 22:4722:47, 12 June 2009 diff hist +7 NanoBio: Prep for Electrocompetent cells →Small-scale prep
• 22:4722:47, 12 June 2009 diff hist +48 NanoBio: Bacterial Transformation using Electroporation →Simultaneous Transformation using Electroporation current
• 22:4622:46, 12 June 2009 diff hist +47 NanoBio: Protocol for gene knockout →Colony PCR for scar
• 22:4422:44, 12 June 2009 diff hist +1,800 NanoBio: Prep for Electrocompetent cells →Large-Scale prep
• 22:2922:29, 12 June 2009 diff hist −90 NanoBio: Prep for Electrocompetent cells →Small-scale prep
• 22:2722:27, 12 June 2009 diff hist +36 NanoBio: Prep for Electrocompetent cells No edit summary
• 22:2622:26, 12 June 2009 diff hist −19 NanoBio: Prep for Electrocompetent cells →Large-scale prep
• 22:2622:26, 12 June 2009 diff hist −18 NanoBio: Prep for Electrocompetent cells →Small-scale prep
• 22:2522:25, 12 June 2009 diff hist +1,430 NanoBio: Prep for Electrocompetent cells →Small-scale prep
• 22:2322:23, 12 June 2009 diff hist +163 N NanoBio: Prep for Electrocompetent cells New page: =Small-scale prep= =Large-scale prep= *'''~~~~''': Return to Protocols.
• 22:2222:22, 12 June 2009 diff hist +80 NanoBio:Protocols →Sub-cloning
• 22:2122:21, 12 June 2009 diff hist −1,369 NanoBio: Bacterial Transformation using Electroporation →Simultaneous Transformation using Electroporation
• 22:1722:17, 12 June 2009 diff hist +1 NanoBio: Protocol for gene knockout →Colony PCR for scar
• 22:1722:17, 12 June 2009 diff hist +357 NanoBio: Protocol for gene knockout →Colony PCR for scar
• 22:1322:13, 12 June 2009 diff hist +45 NanoBio: Protocol for gene knockout →Verify deletion with colony PCR
• 22:1222:12, 12 June 2009 diff hist +12 NanoBio: Protocol for gene knockout →PCR verify deletion
• 22:1122:11, 12 June 2009 diff hist −2 NanoBio: Protocol for gene knockout →Glycerol stocks to keep
• 22:1122:11, 12 June 2009 diff hist +485 NanoBio: Protocol for gene knockout →Remove antibiotic resistance with pCP20
• 20:3120:31, 12 June 2009 diff hist +6 NanoBio: Protocol for gene knockout →Remove antibiotic resistance with pCP20
• 20:3020:30, 12 June 2009 diff hist 0 NanoBio: Protocol for gene knockout →PCR verify deletion
• 20:3020:30, 12 June 2009 diff hist +92 NanoBio: Protocol for gene knockout →PCR verify deletion
• 20:2920:29, 12 June 2009 diff hist +6 NanoBio: Protocol for gene knockout →Electroporate linear PCR product into electrocompetent cells