Sosnick:Protocols

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Protein

Expression and Purification

Protein Expression

Calmodulin affinity purification

His-tag purification

Maltose Binding Protein tag purification

Purifying Ubiquitin

Purifying ctAcP

Chemical Synthesis and Modification

Free Sulfhydryl Determination

Solid Phase Peptide Synthesis

Cyanogen Bromide Cleavage of Ubiquitin

Analysis

Bis-tris SDS-PAGE

Sosnick:Rapid Coomassie Staining

Nucleic Acid

Agarose gel electrophoresis

Designing primers

PCR

PNK Treatment of DNA Ends

CIPing the vector

Ethanol precipitation of nucleic acids

DNA Ligation

Site-directed mutagenesis

Overlap extension PCR

E. coli

Chemically Competent cells (TSS)

Electrocompetent cells