Agenda Items
Alex's work
- JAX
- JAX data getting organized
- Alex may take a stab at editing R script
- Alex will try to set up scanner
- PM
- Alex will update PM_baso data
- received PM -- will review what to do with it
- how is it resuspended for tissue culture -- Wayne will ask EM
- R03
- 2/4 experiments good
- repeat next week with 1X DHR
- Carbo
- alex will set up new compensation controls
- alex will create a FJ analysis template file
- some recent samples have shown response to carbo as per AM
Bert's work
- Ben Medoff's PCR gives better results -- dramatically lower Ct values across the board
- Despite this, CPE response looks weak -- one new lot of StemSpan versus old
- somewhat stronger in X-vivo (n=1)
- ? of whether only CPE response or other pathways are also suppressed
- perhaps we need fresh CPE?
- some hope in a poor quality experiment that ALDH inducing activity is fractionating
- WS will contact Luda about getting a little aliquot
Action Items
- Wayne will talk to Ben about using qPCR more regularly
- Bert will compare LPS response
- run fraction screening experiment on Ben's machine
- repeat fraction screening experiment with pooled fractions
- get larger column for bigger run
- contact Steve about using HPLC
Other Discussion
Things To Do
|
ShreffLab Staff Meetings
Main project page
Previous entry Next entry
