Shreffler:Notebook/R03 Baso/2010/07/29

Basophil Project

Basophil Sensitization (8) - 7/30/10

Patient was already sensitized to rDerp2, as with (7)
Sub-optimal/heterogeneous antibody staining, possibly due to high cell density (clumps were present in almost every wash step, as well as in final volume run in flow)
Labeling discrepancy - rows 2-4 (H:P 1:99, 50:50, 99:1) were labelled in reverse order on FACSDiva, but I'm 90% sure that H:P 1:99, 50:50, 99:1 was correct order