Shreffler:Notebook/R03 Baso/2010/08/04

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Basophil Sensitization (9) - 8/4/10

  • Had starting cell count of approx. 10 million PBMCs per sample (360 million total)
  • Running low on H12 and P4E (rDerp2-specific IgE), so used half the volume described in protocol (instead of 80uL H12 + 80uL P4E + 640 uL medium + 800 uL cells for 1600 uL total, used 40+40+320+400=800)
  • Cells didn't clump as strongly as in prior BasoSens experiments (pellets seemed smaller, too) - then again, I used over 10 millions PBMCs per tube (between 10-20 million)

Flow Analysis

  • Basos were nonresponding...ugh
  • Event rate was good
  1. Media:BasoSens09_20100804.pdf
  2. Media:BasoSens09_20100804-Table.txt
  3. Media:BasoSens09_20100804-Table.pdf