SSC

SSC buffer is named after its ingredients Saline (sodium chloride) and Sodium Citrate. It is used as a buffer for blotting, hybridisation, and stringency washes in Northern and Southern blots as well as DNA microarray.

1x SSC buffer

• 150mM NaCl
• 15mM sodium citrate

20x SSC

• 800ml dH2O (RNase free if required)
• 175.3g NaCl (3M)
• 88.2g trisodium citrate (NaCit; 300mM; e.g. S4641)
• adjust the pH to 7.0 with a few drops of 1M HCl
• adjust the volume to 1L with dH2O
• sterilize by autoclaving

using stock solutions

• 3M NaCl
• 300 mM sodium citrate, pH 7.0

protocols using SSC

• Dahlquist:DNA Microarray Protocol
• Endy:Northern Blot, 32P End-Labeled Probes
• 20.109(S07): cDNA synthesis and microarray
• 20.109(F08): Mod 2 Day 7 cDNA synthesis and microarray

See also

• Wikipedia: SSC buffer