SEED/2010/Schedule

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[[../Overview/]] of the main lab project

Class Date Agenda Homework Lab Protocol
[[../Day 1/]] Feb. 6 safety training, training lab
  • Homework #1: doc pdf
    • read comic
    • iGEM Questions
    • Ideas (3-5) for what you want to engineer biology to do
    • Training lab questions
    • Data Analysis
    • Pre-Lab
Lab Day #1: Serial Dilutions pdf doc
[[../Day 2/]] Feb. 20 SynBio Overview, sterile technique, colony streaking, antibiotic resistance Lab Day #2: Plate Streaking pdf doc
[[../Day 3/]] Feb. 27 miniprep, digest
  • Lab Day #3: Plasmid Mini-Prep pdf doc
  • Restriction Digest pdf doc
[[../Day 4/]] Mar. 6 PCR and gel
[[../Day 5/]] Mar. 13 gel purification: pdf docx Lab Day #5: Gel Purification pdf doc
[[../Day 6/]] Mar. 20 ligation, transformation Lab Day #6: Ligation pdf doc
[[../Day 7/]] Mar. 27 prep for sequencing, glycerol

final project notes: Board1.jpg Board2.jpg Board3.jpg Board4.jpg Board5.jpg Board6.jpg

Lab Day #7: Transformation pdf doc
[[../Day 8/]] Apr. 3 β-gal assays, growth curves, viable cell titer, analyze sequencing
  • Homework #8: TBA
  • [[../Sequencing|Sequence results]]
  • [[../Sequences|Sequences]]
Lab Day #8: Beta-Galactosidase: Part I pdf doc
[[../Day 9/]] Apr. 17 device characterization and composability
  • Homework #9: TBA
Lab Day #9: Beta-Galactosidase: Part II pdf doc
[[../Day 10/]] May 1 data analysis, finish projects
  • Final: TBA
Final Lab: pdf