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- Trying mutagenic PCR of E0240.
- Used 17 cycles. Don't know what # is optimal. Stratagene recommends 12, but people seem to use ~18.
- Too many cycles -> more junk DNA.
- Digesting with DpnI. Felix suggested digesting for >2h.
- Later will try re-transforming into another set of competent Top10 cells.
- Should make more MG1655.
- Re-tried yesterday's transformations into Top10s.
- O/n of MG1655 is growing.