Rao:Protocols

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Rao Lab

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General Techniques

  • Colony PCR Verification
  • Enzymatic Inverse PCR (EI-PCR)
  • Mega-Primer
  • Restriction Digest
  • DNA Ligation
  • Beta-galactosidase Assay
  • Tecan Safire2

E. coli/S. typhimurium specific

  • DNA Transformation
  • Transduction
  • One-step Gene Distruption
  • CRIM Plasmid Integration

B. subtilis specific

  • B. subtilis transformation

Media Preparation

  • LB
  • SOB/SOC
  • M9/M9c
  • Tryptone Broth (TB)
  • Penassay Broth
  • Motility Plates

If your interested in more recipes go here

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  • This page was last edited on 16 December 2010, at 19:00.
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