Quint Lab:yeast western blot

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western blot yeast spin down the over night culture 2 min 10000 rpm pour off the SN add 2x Lämmli: 50 – 100 µl → vortex boil for 5 min → chill on ice spin down 30 s top speed→ chill on ice 15 µl on gel 2 ml over night culture (selective medium) @ 30°C pGBKT7: α-c-myc (1:5000) pGADT7: α-HA (1:5000) pJG4-5/pJCL: α-HA (1:5000) pEG202: α-LexA