Quint Lab:kanamycin selection of transgenic seeds

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kanamycin selection of transgenic seeds Selection of transformed Arabidopsis (Kanamycin Selection) Sterilize seeds and stratify them for 2 d at 4°C in the dark Sow seeds on ATS + 50 μg/ml Kanamycin Cultivate seedlings for 10 d at 20°C under LD conditions Evaluation: transformed seedlings are resistant to Kanamycin and show green and open, expanded cotyledons untransformed seedlings are sensitive to Kanamycin and therefore cotyledons appear yellow and closed