Quint Lab:enzyme lists

From OpenWetWare
Jump to navigationJump to search

home        research        people        papers        teaching        join us        internal        pictures        contact       


Restriktionsenzyme

enzyme isoschizomeres buffer temperature heat inactivated add BSA add SAM add Oligonucleotide star activity


AatII (?) 1x NEBuffer 4 37°C 65°C 20'
AatII 1x Tango 37°C 65°C 20'
Alw26I BsmAI 1x Tango 37°C 65°C 20'
Alw26I Fast Digest (?) BsmAI 1x Fast Digest 37°C 65°C 20'
AseI VspI NEB3 37°C 65°C 20'
BamHI Buffer BamHI 37°C 80°C 20'
BamHI Fast Digest 1x Fast Digest 37°C 80°C 5'
BauI BssSI 1 x Tango 37°C 65°C 20'
BbvI BseXI 1x NEBuffer 2 37°C 65°C 20'
BbsI Fast Digest BpiI 1x Fast Digest 37°C 65°C 10'
BceAI NEB3 37°C 65°C 20' yes
BcuI SpeI 1x Tango 37°C no
BfuI BciVI 1x Buffer BfuI 37°C 80°C 20'
BpiI BbsI 1x Buffer G 37°C 65°C 20'
BglII Fast Digest 1x Fast Digest 37°C no
BseGI FokI 1x Tango 55°C 80°C 20'
BseMII BspCNI 1x Tango 55°C 80°C 20' yes
BseNI BsrI 1x Buffer B 65°C 80°C 20'
BseRI 1x NEBuffer 4 37°C 65°C 20'
Bsp119I BstBI 1x Tango 37°C 80°C 20'
Bsp1407I Fast Digest BsrGI 1x Fast Digest 37°C 80°C 10'
BspOI BmtI 1x Buffer O 37°C 80°C 20'
BveI BspMI 1x Buffer O 37°C 65°C 20' yes *
Cfr10I BsrFI Buffer Cfr10I 37°C no *
DpnI Fast Digest BsrFI 1x Fast Digest 37°C 80° 5' *
EagI-HF Eco52I 1x NEBuffer 4 37°C 65°C 20' *
Eam1104I EarI 1x Tango 37°C 65°C 20'
Eco31I Fast Digest 1x Fast Digest 37°C 65°C 5'
Eco32I EcoRV 1x Buffer R 37°C 80°C 20'
Eco47I AvaII 1x Buffer R 37°C 65°C 20'
Eco147I StuI 1x Buffer B 37°C 80°C 20'
EcoRI Buffer EcoRI 37°C 65°C 20'
EcoRI Fast Digest 1x Fast Digest 37°C 80°C 5'
EcoRV Eco32I NEB3 37°C 80°C 20' yes
EcoRV Fast Digest 1x Fast Digest 37°C no
FspI NsbI 1x NEBuffer 4 37°C 65°C 20'
HindIII NEB2 37°C 65°C 20'
Hin6I HinP1I 1x Tango 37°C no
Hpy188I NEB4 37°C 65°C 20'
HpyF3I DdeI 1x Tango 37°C 65°C 20'
HpyF10VI MwoI 1x Tango 37°C 80°C 20'
KpnI ReACT4 37°C
KpnI Fast Digest 1x Fast Digest 37°C 80°C 5'
Kpn2I BspEI 1 x Tango 55°C 80°C 20'
KspAI HpaI 1x Buffer B 37°C 65°C 20'
MboI 1x Buffer R 37°C 65°C 20'
MboII 1x Buffer B 37°C 65°C 20'
MnlI 1x Buffer G 37°C 65°C 20'
MvaI BstNI 1x Buffer R 37°C no
NcoI 1x Tango 37°C 65°C 20'
NcoI Fast Digest 1x Fast Digest 37°C 65°C 15'
NdeI Fast Digest 1x Fast Digest 37°C 65°C 5'
PacI (?) NEB1 37°C 65°C 20' yes
PaeI SphI 1x Buffer B 37°C 65°C 20'
PciI PscI NEB3 37°C 80°C 20' yes
PsyI Tth111I 1x Buffer B 37°C 80°C 20'
PvuII NEB2 37°C no
SacI Buffer SacI 37°C 65°C 20'
SacII Cfr42I NEB4 37°C 65°C 20'
SalI SuRE/Cut Buffer H 37°C *
SapI 1x NEBuffer 4 37°C 65°C 20'
SmaI 1x Tango 30°C 65°C 20'
ScrFI Bme1390I NEB4 37°C 65°C 20'
SpeI Fast Digest (?) BcuI 1x Fast Digest 37°C no
TaqI Buffer TaqI 65°C no
VspI AseI 1x Buffer O 37°C 65°C 20'
XhoI 1x Buffer R 37°C 80°C 20'
XhoI Fast Digest 1x Fast Digest 37°C 80°C 5'
(?) = Achtung sehr wenig

DNA-modifying

enzym in stock? supplier, bestnr. jahr
T4 DNA ligase y z
RevertAid M-MuLV Reverse Transcriptase ja Fermentas, EP0442 2008
x y z
x y z