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NanoDrop Procedure:

Before every set:

  1. Clean with Ethanol.
  2. Calibrate with 1 μL water (distilled).
  3. Clean.
  4. Place 1 μL distilled water on drop again & hit Blank button.
    • This sets background, since we diluted in water. If you dilute in something else, use that instead of water here.
  5. Clean.
  6. Add 1 μL sample (mix first).
  7. Enter name/identifier in ID box and hit Measure button.

Other, more complete procedure can be found here.