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Before every set:
- Clean with Ethanol.
- Calibrate with 1 μL water (distilled).
- Place 1 μL distilled water on drop again & hit Blank button.
- This sets background, since we diluted in water. If you dilute in something else, use that instead of water here.
- Add 1 μL sample (mix first).
- Enter name/identifier in ID box and hit Measure button.
Other, more complete procedure can be found here.