Michael R. Pina Week 2
Week 2
(a) and (b)
| Allele/Color | Change(s) in amino acid sequence | Change(s) in DNA sequence |
| Green | TyrTrp | ACTGGC |
| Red | PheCys | TCTGTC |
| Blue | TyrCys | ACTGTC |
| Yellow | TrpCys | GGTGTC |
| White | Any other combination | Any other combination |
Are all the white alleles the same?
No, there are only a few amino acid sequences that are responsible for pigmentation (near base pair 80); if it is any other of the amino acids then the resultant flower will be white.
(c)
Green-1
Upper (green)
CAGCTATAACCGAGATTGATGTCTAGTGCGATAAGCCCCAAAGATCGGCACATTTTGTGCGCTATACAAAGGTTAGTGTACTGGCGGCAGTAGTAGGGGGCGT
Lower (green)
CAGCTATAACCGAGATTGATGTCTAGTGCGATAAGCCCCAAAGATCGGCACATTTTGTGCGCTATACAAAGGTTAGTGTACTGGCGGCAGTAGTAGGGGGCGT
Green-2
Upper (blue)
CAGCTATAACCGAGATTGATGTCTAGTGCGATAAGCCCCAAAGATCGGCACATTTTGTGCGCTATACAAAGGTTAGTGTACTGTCGGCAGTAGTAGGGGGCGT
Lower (yellow)
CAGCTATAACCGAGATTGATGTCTAGTGCGATAAGCCCCAAAGATCGGCACATTTTGTGCGCTATACAAAGGTTAGTGTGGTGTCGGCAGTAGTAGGGGGCGT
Red
Upper (red)
CAGCTATAACCGAGATTGATGTCTAGTGCGATAAGCCCCAAAGATCGGCACATTTTGTGCGCTATACAAAGGTTAGTGTTCTGTCGGCAGTAGTAGGGGGCGT
Lower (white)
CAGCTATGACCGAGATTGATGTCTAGTGCGATAAGCCCCAAAGATCGGCACATTTTGTGCGCTATACAAAGGTTAGTGTTCTGTCGGCAGTAGTAGGGGGCGT
White
Upper (white)
CAGCTATAACCGAGATTGATGTCTAGTGCGATAAGCCCCAAAGATCGGCACATTTTGTGCGCTATACAAAGGTTAGTGGTCTGTCGGCAGTAGTAGGGGGCGT
Lower (white)
CAGCTATAACCGAGATTGATGTCTAGTGCGATAAGCCCCAAAGATCGGCACATTTTGTGCGCTATACAAAGGTTAGTGGTCTGTCGGCAGTAGTAGGGGGCGT
By an eye-ball comparison it is easy to note that all of the DNA sequences are identical until base pair 79.
(d)
To create a true breeding purple flower, a mutant was created by adding the DNA sequence "ACTTCT" starting at base pair 79. "ACTTCT" codes for the amino acid sequence Tyr-Phe. It was found that Tyr causes blue pigmentation and Phe cause red pigmentation, and when placed together they result in a purple mutant. For more detail on this process, take a look at my lab notebook.
