McClean: Tris Buffer

Overview

Tris-Buffer

Materials

• MilliQ Water
• TrisBase
• HCl

Procedure

Tris-Buffer can prepared at various desired pH values. This procedure covers how to prepare a Tris-Buffer of pH 7.0, 7.5, and 8.0 respectively. Each protocol prepares 2L of solution. In addition, each Tris-Buffer requires the addition of 242.2 g of TrisBase.

First, add 242.2 g of TrisBase to 2L of MilliQ Water. Then in order to prepare desired pH add 37% HCL accordingly:

• pH 7.0, 150-155mL HCl
• pH 7.5, 120-125mL HCl
• pH 8.0, 80-85mL HCl

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References

http://web.stanford.edu/group/gozani/cgi-bin/gozanilab/wp-content/uploads/2014/01/Buffer-Preparation.pdf

Contact

• Megan N McClean 14:01, 20 July 2011 (EDT)

or instead, discuss this protocol.