McClean: TE 10x

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How to make TE 10x.TE is used for storing DNA and RNA. All necessary items are found in 3005A.


  1. 1M Tris pH 7.5 or 8.0- you can use HCl to change the pH as necessary.
  2. .5M EDTA pH 8.0
  3. ddH2O (we use Milli-Q water)


  • Measure out and pour 100ml of Tris into a CLEAN 1L glass bottle.
  • Measure out and pour 20ml of EDTA into the 1L glass bottle.
  • Fill to 1000ml with Milli-Q water.
  • Label the new TE and parafilm the Tris and EDTA.

Other Information

Ask Megan if you have any questions!


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