McClean: TAE 50X
2M Tris Base and 50nM EDTA
Glacial Acetic Acid
For 2L of Buffer:
Dissolve 484.4g Tris Base in around 1L of water
Add 114.2mL glacial acetic acid and 200mL 0.5M EDTA
Transfer solutions to graduated cylinders to volumize to 2L
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
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- Megan N McClean 14:01, 20 July 2011 (EDT)
or instead, discuss this protocol.