Matt Gethers/CRI, Thailand/Labwork/Phosphatase Treatment

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Alkaline Phosphatase Treatment (SAP Enzyme)

Reagent Volume Notes
Digested Vector 8.5 μl
SAP Buffer 1 μl
SAP Enzyme 0.5 μl
Total 10 μl

Incubate mix at 37oC for 30 minutes, then inactivate at 65oC for 15 minutes.

Run Notes

7.22.08

Ran protocol as written to dephosphorylate pKn002.P1 cut with SphI and HindIII.