Matt Gethers/CRI, Thailand/Labwork/Phosphatase Treatment
Alkaline Phosphatase Treatment (SAP Enzyme)
|Digested Vector||8.5 μl|
|SAP Buffer||1 μl|
|SAP Enzyme||0.5 μl|
Incubate mix at 37oC for 30 minutes, then inactivate at 65oC for 15 minutes.
Ran protocol as written to dephosphorylate pKn002.P1 cut with SphI and HindIII.