Matt Gethers/CRI, Thailand/Labwork/PCRs/Screening for Presence of Lox/Gen Cassette in pKn004/8.5.08 Master mix

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8.5.08 PCR Master

Reagent Volume/Amount
Colony Suspension 8.5 μl, but don't add to master
Taq 0.85 μl
M13 for 6.8 μl 1 μM Stock
BT1188 6.8 μl 1 μM Stock
dNTPs 1.7 μl 10 mM Stock
DMSO 4.25 μl
Buffer 8.5 μl
Mg2+ 4.25 μl
dH20 43.35 μl
Total 50 μl

Aliquotted 9 μl to each of 8 tubes, 7 colony PCRs, 1 negative control using 0.5 μl pKn004.P1. Added 1 μl colony suspension to the rest.