Lysis Methods

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100702

• first complex cell lysate prep'
  • lysed with 2% SDS, 100mM DTT, in 1X PBS (approx pH = 7.4)
  • 2 mL per 15cm dish
• Split to multiple samples:
  • Added 15% glycerol, then frozen @ -80
  • Frozen @ -80
  • Frozen @ -20
  • 15% glycerol, frozen @ -20