Kubke Lab:Research/CND/Records2010-2011Summer/RC012
Cranial Nerve Development | Experiment |
Embryo details
Species: Gallus gallus domesticus
Embryo Name: RC001
Embryo stage: ST29
Staging description: Staged by Reuben according to the Hamburger and Hamilton (1951) staging system. (
Notebook entry 10th January 2011). Staging has not been confirmed.
- Beak beginning to form (>ST26).
- 3 visible toes have formed (>ST25).
- Visceral cleft not visible (>ST26).
- Heart enclosed.
- Beak shape matches ST28-ST29.
- Neck region is thinner than ST28 (therefore ST29).
Fixation:PFA
Cryoprotection: None.
Material label and storage: Stored in vial filled with PFA labeled RC-012 and placed in a container labeled 'Reuben's staged embryos' next to the dissection microscope.
Experiment details
Objective: To use as a practice embryo for staging.
Procedure: Staged the embryo by observing the embryo under a dissection microscope and comparing its morphological properties with those described in the Hamburger and Hamilton (1951) paper whilst looking at our own compilation of images organized by stage.
Comments:
Results
Embryo is approximately ST29
Images
Summary
This embryo may be used in further experiments as approximately a ST29 embryo however confirmation is needed.