Kubke Lab:Research/CND/Records2010-2011Summer/RC010

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Cranial Nerve Development Experiment

Embryo details

Species: Gallus gallus domesticus
Embryo Name: RC010
Embryo stage: ST25
Staging description: Staged by Reuben according to the Hamburger and Hamilton (1951) staging system. (Notebook entry 10th January 2011 ). Staging has not been confirmed.

  • Too grooves present but not distinct (<ST26).
  • Tail region segmented.
  • Visceral clefts still visible (<ST26).
  • Shape of the hindbrain has a sligh groove which resembles the images for ST25.

Fixation: PFA
Cryoprotection: None
Material label and storage: Stored in vial filled with PFA labeled RC-010 and placed in a container labeled 'Reuben's staged embryos' next to the dissection microscope.

Experiment details

Objective: To use as a practice embryo for staging.
Procedure: Staged the embryo by observing the embryo under a dissection microscope and comparing its morphological properties with those described in the Hamburger and Hamilton (1951) paper whilst looking at our own compilation of images organized by stage.


Embryo is approximately ST25.



This embryo may be used in further experiments as approximately a ST25 embryo however confirmation is needed.