Kubke Lab:Research/CND/Records2010-2011Summer/MH004
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| Cranial Nerve Development | Experiment |
Embryo details
Species:Gallus gallus domesticus
Embryo Name:MH004
Embryo stage:ST28-29
Staging description:
Fixation:PF
Cryoprotection:No
Material label and storage:Stored in a vial labelled MH004 filled with PF solution.
Experiment details
Objective: To determine what are the best parameters to cut a stage 28 embryo.
Procedure:
Cryostat Sectioning Cryostat settings (for a more detailed protocol visit Kubke_Lab:Cryomicrotomy)
| Cryostat | Histology Lab |
| Knife | |
| Day Cut | 16/12/2010 |
| Knife Angle | 0 |
| Chamber Temp | -16C |
| Object Temp | -16c |
| Glass Slides | Polysine slides |
| Plane of section | Coronal |
| Number of slides | |
| Observations | Fabiana's notes |
(Include in your observations, eg ,were the sections serial, was any section lost, was quality assessed, etc)
Comments: Kubke_Lab:/Notebook/Cranial_nerve_development/2010/12/16#Cryostat_sectioning
Results
- Nissl stained
