Koch Lab:Protocols/GPEM

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GPEM is PEM + 1 mM GTP. PEM is BRB80 and also referred to as "general tubulin buffer."

As of June, 2009, we are making GPEM as follows:

  • 98 parts PEM
  • 2 parts 50 mM GTP

For example, on 6/15/2009, Linh is making 300 microliters of GPEM by adding:

  • 294 microliters PEM (from cytoskeleton)
  • 6 microliters of GTP