Keck Subbasement:Equipment

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Available equipement

Rough list of equipment which is currently available:

Small Thermal cycler: Can do gradients. Gel equipment: Gel scanner with attached computer. Image quality is very bad though. Polyacrylimide Gel Box, Temperature Controlled. (Temperature control not set up yet) SPEX (Flourolog 3) For registration on Google Calendars, contact Elisa. Large Eppendorf: Has 2 rotors: One for conicals and One for 1.5 mLs. Limit on Conicals is 4000x RPM. Limit on 1.5 mLs is 15000x RPM. Bench micro/nano-fuge + vortexers Hot plate and stirrer Heat Block Vacufuge -80 Freezer Incubator/Shaker Miscellaneous equipment: Pipetters

Fume hood Pelton and Crane sterilizer - small, still working.

There is also some equipment in nearby instructional labs (mainly Spalding) that we can use over the summer

• Electroporation machine - Spalding or Broad (not sure where)
• Plate reader - Spalding
• -80 freezer space - Spalding

Missing equipment

Here is a list of equipment that the lab is missing:

2008 list

• Balance ($3K)
• Autoclave - this can probably be shared with others in Spalding. A decent-sized autoclave looks like it costs $10-20K. Jared has one that is out for repair. Chinlin does not currently have an autoclave
• Spectroflourimeter ($25K+)
• Spectrophotometer ($??) or nanodrop ($8K) - share with others in Spalding. Check on current spectrophotometer first
• Nanopure (water filtration machine) - a small benchtop unit seems to cost about $3-5K
• Hot plate with stirrer (found one in the lab)
• Ice machine - the one in the subbasement is broken. Jared has one; Chinlin has ordered one => we can probably do without
• Gel imager ($8-10K) - we have the equipment required to image gels by hand; might be useful at some point to get a real imager

2007 list

• Electroporation Machine
• Filters for scope that can see mCherry, cerulean, and YFP
• -80 deg C freezer - available in Spalding
• Fluorimeter - available in Spalding, but might be nice to have one
• Equipment for running polyacrylamide gels

Purchased

• Medium/Large Centrifuge (for minipreps, competent cells, falcon tubes) - purchased in 2007; now sitting in the Spalding instructional laboratory