IGEM:metu/2009/Notebook/wound dressing/2009/08/31

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31.08.2009

1. We made digestions. The following amounts are added to microtubes;

  2 ,            Buffer                    3 µl              
                 Xba1                      3 µl            
                 DNA                      18 µl           
                 water                     6 µl    =30 µl
  BB,            Buffer                    3 µl            
                 EcoR1+Pst1            1.5+1.5 µl           
                 DNA                      20 µl           
                 water                     4 µl    =30 µl

2. Electrophoresis order;

  1)leader / BB control / BB / 2 control / 2

3. Gell extraction for 2 and BB were made.