IGEM:metu/2009/Notebook/wound dressing/2009/08/29

From OpenWetWare
Jump to: navigation, search
Igem-logo-150px.png WOUND DRESSING Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Wound dressing logo.jpg

29.08.2009

1. Isolations of 1,2,3,4,5,6,7,8,9,10,11,BB were made.

2. Digestions of 1 and 2 were made

                                                                            control
   1 ,            Buffer                  5 µl                                2  µl
                  Spe1                    5 µl                                0  
                  DNA                    40 µl                               10 µl
                  water                   0        =50 µl                     8 µl       =20 µl
   
                                                                            control
   2 ,            Buffer                  5 µl                                2 µl
                  Pst1                    5 µl                                0
                  DNA                    40 µl                               10 µl
                  water                   0        =50 µl                     8 µl       =20 µl

3. Electrophoresis order;

 1)leader / c1/  1 / 11 / s1/c2/ 2 / 22/ s2 / leader
 2)leader / c1/  1 / i1 / p1/c2/ 2 / i2/ p2 / leader

4. We couldn't digest.