IGEM:metu/2009/Notebook/wound dressing/2009/08/28
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 WOUND DRESSING
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28.08.20091. Electrophoresis order; 1)leader / 6M / 8M / 6 / 6 / leader 2. We made digestions.The following amounts are added to microtubes; 6M, water 18.4 µl
Buffer 3 µl
Pst1 3 µl
DNA 5.6 µl =30 µl
1st part 2nd part control
8M/D, water 17.9 µl 8 µl 34 µl
Buffer 3 µl 4 µl 4 µl
Pst1 3 µl Xba1 4 µl 0
DNA 6.1 µl =30 µl 24 µl =40 µl 2 µl =40 µl
3. Electrophoresis order; 1)empty/empty/8MD/8M control/leader 4. We couldn't digest.
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