IGEM:metu/2009/Notebook/wound dressing/2009/08/28

From OpenWetWare
Jump to: navigation, search
Igem-logo-150px.png WOUND DRESSING Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Wound dressing logo.jpg

28.08.2009

1. Electrophoresis order;

  1)leader / 6M / 8M / 6 / 6 / leader

2. We made digestions.The following amounts are added to microtubes;

    6M,           water       18.4 µl
                  Buffer       3 µl               
                  Pst1         3 µl
                  DNA          5.6 µl     =30 µl 
                             1st part                   2nd part                 control
    8M/D,         water       17.9 µl                      8 µl                   34 µl
                  Buffer       3 µl                        4 µl                    4 µl
                  Pst1         3 µl               Xba1     4 µl                    0
                  DNA          6.1 µl   =30 µl            24  µl      =40 µl       2 µl        =40 µl

3. Electrophoresis order;

  1)empty/empty/8MD/8M control/leader

4. We couldn't digest.