IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 RNA Decoder/2010/06/30
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June 30, 2010
Today we tried cloning out MicA, MicF, OmpA, OmpF, and Hfq. The procedure we followed was:
A master mix instead of adding each of those amounts to each tube. The master mix was made as such (MM for 5 RXNs):
We also ran controsl for each reaction: one without template, and one without polymerase. In each water replaced the missing material.
The PCR settings were:
We then ran a electrophoresis gel of my PCR results.