IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 RNA Decoder/2010/06/29

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Entry title

Did a Digestion today of pSB1AT3 to test our digestion enzymes. We cut pSB1AT3 with SpeI+XbaI, SpeI, and XbaI. To do this procedure we used:

  • RXN 1 (SpeI)
  • SpeI 1 uL
  • Buffer (2) 5 uL
  • BSA .5 uL
  • DNA (pSB1AT3) 1.5 uL
  • dH2O 42 uL
  • Total= 50 uL
  • RXN 2 (XbaI)
  • XbaI 1 uL
  • Buffer (2) 5 uL
  • BSA .5 uL
  • DNA (pSB1AT3) 1.5 uL
  • dH2O 42 uL
  • Total= 50 uL
  • RXN 3 (SpeI + XbaI):
  • SpeI 1 uL
  • XbaI 1 uL
  • Buffer (2) 5 uL
  • BSA .5 uL
  • DNA (pSB1AT3) 1.5 uL
  • dH2O 41 uL
  • Total= 50 uL

We then ran a gel of the 3 reactions on 1% agarose gel.